Title:
METHOD FOR PRODUCING ORGANOID FROM LUNG EPITHELIAL CELLS OR LUNG CANCER CELLS
Document Type and Number:
WIPO Patent Application WO/2021/241621
Kind Code:
A1
Abstract:
This method for producing an organoid from lung epithelial cells or lung cancer cells includes culturing a specimen containing lung epithelial cells or lung cancer cells in a culture medium. The culture medium includes: 0-10%v/v of extracellular matrix; and a combination of at least one selected from the group consisting of keratinocyte growth factor (KGF), fibroblast growth factor (FGF) 10 and hepatocyte growth factor (HGF), a bone morphogenetic protein (BMP) inhibitor, and TGF-β inhibitor. The culture medium substantially does not include feeder cells.
Inventors:
MORIMOTO MITSURU (JP)
FUJIMURA TAKASHI (JP)
FUJIMURA TAKASHI (JP)
Application Number:
PCT/JP2021/019947
Publication Date:
December 02, 2021
Filing Date:
May 26, 2021
Export Citation:
Assignee:
OTSUKA PHARMA CO LTD (JP)
RIKEN (JP)
RIKEN (JP)
International Classes:
A61P11/00; A61K35/42; A61L27/38; A61P43/00; C12N5/074; C12N5/09; G01N33/15; G01N33/50
Foreign References:
| JP2018503360A | 2018-02-08 | |||
| JP2020511968A | 2020-04-23 | |||
| JP2018110575A | 2018-07-19 | |||
| JP2016513469A | 2016-05-16 | |||
| JP2018503360A | 2018-02-08 |
Other References:
NG-BLICHFELDT JOHN-POUL, SCHRIK ANNEKE, KORTEKAAS ROSA K., NOORDHOEK JACOBIEN A., HEIJINK IRENE H., HIEMSTRA PIETER S., STOLK JAN,: "Retinoic acid signaling balances adult distal lung epithelial progenitor cell growth and differentiation", EBIOMEDICINE, vol. 36, 17 September 2018 (2018-09-17), pages 461 - 474, XP055880302, Retrieved from the Internet
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 515, 6 August 2019 (2019-08-06), pages 579 - 585
PROC. NATL. ACAD. SCI. USA, vol. 106, no. 31, pages 12771 - 12775
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, vol. 58, no. 10, 2010, pages 891 - 901
NATURE COMMUNICATIONS, 2019, pages 103991
J IMMUNOL, vol. 180, 2008, pages 881 - 888
RAPID COMMUNICATIONS, pages L349 - L356
STEM CELLS, vol. 30, 2012, pages 1948 - 1960
BARKAUSKAS CE, J CLIN INVEST, vol. 123, no. 7, 2013, pages 3025 - 36
PROC NATL ACAD SCI USA., vol. 106, no. 31, 4 August 2009 (2009-08-04), pages 12771 - 5
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 515, 6 August 2019 (2019-08-06), pages 579 - 585
PROC. NATL. ACAD. SCI. USA, vol. 106, no. 31, pages 12771 - 12775
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, vol. 58, no. 10, 2010, pages 891 - 901
NATURE COMMUNICATIONS, 2019, pages 103991
J IMMUNOL, vol. 180, 2008, pages 881 - 888
RAPID COMMUNICATIONS, pages L349 - L356
STEM CELLS, vol. 30, 2012, pages 1948 - 1960
BARKAUSKAS CE, J CLIN INVEST, vol. 123, no. 7, 2013, pages 3025 - 36
PROC NATL ACAD SCI USA., vol. 106, no. 31, 4 August 2009 (2009-08-04), pages 12771 - 5
Attorney, Agent or Firm:
YAMAO, Norihito et al. (JP)
Download PDF:
Previous Patent: HOLLOW FIBER MEMBRANE REPLACING METHOD
Next Patent: POWER CONVERSION CIRCUIT, SEMICONDUCTOR DEVICE, AND ELECTRONIC APPARATUS
Next Patent: POWER CONVERSION CIRCUIT, SEMICONDUCTOR DEVICE, AND ELECTRONIC APPARATUS