Title:
METHOD FOR PRODUCING PACEMAKER TISSUE FRAGMENT
Document Type and Number:
WIPO Patent Application WO/2015/025958
Kind Code:
A1
Abstract:
Provided is a method for producing a new three-dimensional tissue fragment which can function as a biological pacemaker. This method for producing a pacemaker tissue fragment includes the formation of a three-dimensional tissue fragment obtained by laminating pacemaker cells derived from pluripotent stem cells. The three-dimensional tissue fragment includes pacemaker cells and an extracellular matrix component, and the formation of the three-dimensional tissue fragment includes: laminating coated cells obtained by coating the surface of pacemaker cells with a membrane containing an extracellular matrix component, and culturing the resultant cells; or laminating the cell layer of pacemaker cells alternately with a layer containing the extracellular matrix component, and culturing the resultant cells.
Inventors:
AKASHI MITSURU (JP)
MATSUSAKI MICHIYA (JP)
ITO HIROSHI (JP)
NAKAMURA KAZUFUMI (JP)
SAITO YUKIHIRO (JP)
MATSUSAKI MICHIYA (JP)
ITO HIROSHI (JP)
NAKAMURA KAZUFUMI (JP)
SAITO YUKIHIRO (JP)
Application Number:
PCT/JP2014/072031
Publication Date:
February 26, 2015
Filing Date:
August 22, 2014
Export Citation:
Assignee:
UNIV OSAKA (JP)
UNIV OKAYAMA NAT UNIV CORP (JP)
UNIV OKAYAMA NAT UNIV CORP (JP)
International Classes:
C12N5/07; A61L27/00; C12N5/071; C12N5/0735; C12N5/10
Domestic Patent References:
| WO2011040469A1 | 2011-04-07 | |||
| WO2008112323A1 | 2008-09-18 | |||
| WO2005028638A1 | 2005-03-31 |
Foreign References:
| JP2007228921A | 2007-09-13 | |||
| JP2012115254A | 2012-06-21 | |||
| JP2013520168A | 2013-06-06 | |||
| JP2006523091A | 2006-10-12 |
Other References:
SHIMIZU, T. ET AL.: "Fabrication of Pulsatile Cardiac Tissue Grafts Using a Novel 3- Dimensional Cell Sheet Manipulation Technique and Temperature-Responsive Cell Culture Surfaces", CIRC. RES., vol. 90, 2002, pages E40 - E48
SAITO, Y. ET AL.: "Establishment of hyperpolarization-activated cyclic nucleotide- gated potassium channel 4-overexpressing mouse embryonic stem cell -derived cardiomyocytes as a candidate for a biological pacemaker", EUROPEAN HEART JOURNAL, vol. 34, no. SUPPL., 1 August 2013 (2013-08-01), pages 278 - 279
TOHYAMA, S. ET AL.: "Distinct Metabolic Flow Enables Large-Scale Purification of Mouse and Human Pluripotent Stem Cell -Derived Cardiomyocytes", CELL STEM CELL, vol. 12, 3 January 2013 (2013-01-03), pages 127 - 137, XP055175411, DOI: doi:10.1016/j.stem.2012.09.013
KEHAT, I. ET AL.: "Electromechanical integration of cardiomyocytes derived from human embryonic stem cells", NATURE BIOTECHNOLOGY, vol. 22, no. 10, October 2004 (2004-10-01), pages 1282 - 1289
ZIMMERMANN, W.-H. ET AL.: "Engineered heart tissue grafts improve systolic and diastolic function in infarcted rat hearts", NATURE MEDICINE, vol. 12, no. 4, April 2006 (2006-04-01), pages 452 - 458, XP055083857, DOI: doi:10.1038/nm1394
SAITO, Y. ET AL.: "Establishment of hyperpolarization-activated cyclic nucleotide- gated potassium channel 4-overexpressing mouse embryonic stem cell -derived cardiomyocytes as a candidate for a biological pacemaker", EUROPEAN HEART JOURNAL, vol. 34, no. SUPPL., 1 August 2013 (2013-08-01), pages 278 - 279
TOHYAMA, S. ET AL.: "Distinct Metabolic Flow Enables Large-Scale Purification of Mouse and Human Pluripotent Stem Cell -Derived Cardiomyocytes", CELL STEM CELL, vol. 12, 3 January 2013 (2013-01-03), pages 127 - 137, XP055175411, DOI: doi:10.1016/j.stem.2012.09.013
KEHAT, I. ET AL.: "Electromechanical integration of cardiomyocytes derived from human embryonic stem cells", NATURE BIOTECHNOLOGY, vol. 22, no. 10, October 2004 (2004-10-01), pages 1282 - 1289
ZIMMERMANN, W.-H. ET AL.: "Engineered heart tissue grafts improve systolic and diastolic function in infarcted rat hearts", NATURE MEDICINE, vol. 12, no. 4, April 2006 (2006-04-01), pages 452 - 458, XP055083857, DOI: doi:10.1038/nm1394
Attorney, Agent or Firm:
IKEUCHI SATO & PARTNER PATENT ATTORNEYS (JP)
Patent business corporation Ikeuchi and Sato and partners (JP)
Patent business corporation Ikeuchi and Sato and partners (JP)
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