Title:
METHOD FOR PRODUCING PANCREATIC HORMONE-PRODUCING CELL, PANCREATIC HORMONE-PRODUCING CELL, AND DIFFERENTIATION/INDUCTION PROMOTER
Document Type and Number:
WIPO Patent Application WO/2013/176249
Kind Code:
A1
Abstract:
Provided are: a method for producing (a method for differentiating/inducing) a pancreatic hormone-producing cell, which enables the highly efficient differentiation/induction of a pluripotent stem cell or a pancreatic tissue stem/progenitor cell into a pancreatic hormone-producing cell; a pancreatic hormone-producing cell produced by the method; and a differentiation/induction promoter for use in the production method.
The method for producing a pancreatic hormone-producing cell according to the present invention is characterized in that a specific differentiation/induction promoter is added to a culture medium in the process of differentiating/inducing of a pluripotent stem cell or a pancreatic tissue stem/progenitor cell into the pancreatic hormone-producing cell. The differentiation/induction promoter to be used is: a polypeptide which comprises an amino acid sequence encoded by DNA comprising the nucleotide sequence represented by SEQ ID NO: 1 or a variant thereof; or a culture supernatant of a cell into which DNA comprising the nucleotide sequence represented by SEQ ID NO: 1 or DNA capable of hybridizing with DNA comprising a nucleotide sequence complementary to the aforementioned DNA under stringent conditions is integrated as a foreign gene.
More Like This:
| WO/2023/286819 | METHOD FOR MANAGING QUALITY OF SPECIFIC CELLS, AND METHOD FOR MANUFACTURING SPECIFIC CELLS |
| JP2019514390 | How to form renal tubules |
| WO/1991/001393 | ARTIFICIAL BIOLOGICAL MEMBRANE |
Inventors:
TOYOSHIMA HIDEO (JP)
OKAZAKI YASUSHI (JP)
YOKOO TOMOTAKA (JP)
SUGAWARA IZUMI (JP)
OKAZAKI YASUSHI (JP)
YOKOO TOMOTAKA (JP)
SUGAWARA IZUMI (JP)
Application Number:
PCT/JP2013/064469
Publication Date:
November 28, 2013
Filing Date:
May 24, 2013
Export Citation:
Assignee:
UNIV SAITAMA MEDICAL (JP)
International Classes:
C12N5/071; C12N15/09
Domestic Patent References:
| WO2009013794A1 | 2009-01-29 | |||
| WO2007103282A2 | 2007-09-13 | |||
| WO2005063971A2 | 2005-07-14 | |||
| WO2009048675A1 | 2009-04-16 | |||
| WO2007051038A2 | 2007-05-03 | |||
| WO2006108361A1 | 2006-10-19 | |||
| WO2008066199A1 | 2008-06-05 | |||
| WO2006020919A2 | 2006-02-23 |
Foreign References:
| JP2007209214A | 2007-08-23 |
Other References:
D'AMOUR, K.A. ET AL.: "Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells", NATURE BIOTECHNOLOGY, vol. 24, 2006, pages 1392 - 1401, XP002650232
KUNISADA, Y. ET AL.: "Small molecules induce efficient differentiation into insulin- producing cells from human induced pluripotent stem cells", STEM CELL RESEARCH, vol. 8, no. 2, March 2012 (2012-03-01), pages 274 - 284, XP055094959
JIANG, W. ET AL.: "In vitro derivation of functional insulin-producing cells from human embryonic stem cells", CELL RESEARCH, vol. 17, 2007, pages 333 - 344, XP002455184
D'AMOUR,K.A. ET AL., NATURE BIOTECHNOLOGY, vol. 24, 2006, pages 1392 - 1401
WEI JIANG ET AL., CELL RESEARCH, vol. 17, 2007, pages 333 - 344
MIYAZAKI,S ET AL., DIABETES, vol. 53, 2004, pages 1030 - 1037
YUYA KUNISADA ET AL., STEM CELL RESEARCH, vol. 8, 2012, pages 274 - 284
JONSSON, J. ET AL., NATURE, vol. 371, 1994, pages 606 - 609
OFFIELD, M. F. ET AL., DEVELOPMENT, vol. 22, 1996, pages 983 - 995
MARK, D. F. ET AL., PROC. NATL. ACAD. SCI. USA, vol. 81, 1984, pages 5662 - 5666
ZOLLER, M. J. ET AL., NUCLEIC ACIDS RESEARCH, vol. 10, 1982, pages 6487 - 6500
WANG, A. ET AL., SCIENCE, vol. 224, pages 1431 - 1433
DALBADIE-MCFARLAND, G. ET AL., PROC. NATL. ACAD. SCI. USA, vol. 79, 1982, pages 6409 - 6413
"Molecular Cloning: A Laboratory Manual, 2nd Ed.,", 1989, COLD SPRING HARBOR LABORATORY
J. BIOL. CHEM., vol. 277, no. 34, 2002, pages 30998 - 31004
NISHIMURA, K. ET AL., J. BIOL. CHEM., vol. 286, 2011, pages 4760 - 4771
KUNISADA, Y. ET AL.: "Small molecules induce efficient differentiation into insulin- producing cells from human induced pluripotent stem cells", STEM CELL RESEARCH, vol. 8, no. 2, March 2012 (2012-03-01), pages 274 - 284, XP055094959
JIANG, W. ET AL.: "In vitro derivation of functional insulin-producing cells from human embryonic stem cells", CELL RESEARCH, vol. 17, 2007, pages 333 - 344, XP002455184
D'AMOUR,K.A. ET AL., NATURE BIOTECHNOLOGY, vol. 24, 2006, pages 1392 - 1401
WEI JIANG ET AL., CELL RESEARCH, vol. 17, 2007, pages 333 - 344
MIYAZAKI,S ET AL., DIABETES, vol. 53, 2004, pages 1030 - 1037
YUYA KUNISADA ET AL., STEM CELL RESEARCH, vol. 8, 2012, pages 274 - 284
JONSSON, J. ET AL., NATURE, vol. 371, 1994, pages 606 - 609
OFFIELD, M. F. ET AL., DEVELOPMENT, vol. 22, 1996, pages 983 - 995
MARK, D. F. ET AL., PROC. NATL. ACAD. SCI. USA, vol. 81, 1984, pages 5662 - 5666
ZOLLER, M. J. ET AL., NUCLEIC ACIDS RESEARCH, vol. 10, 1982, pages 6487 - 6500
WANG, A. ET AL., SCIENCE, vol. 224, pages 1431 - 1433
DALBADIE-MCFARLAND, G. ET AL., PROC. NATL. ACAD. SCI. USA, vol. 79, 1982, pages 6409 - 6413
"Molecular Cloning: A Laboratory Manual, 2nd Ed.,", 1989, COLD SPRING HARBOR LABORATORY
J. BIOL. CHEM., vol. 277, no. 34, 2002, pages 30998 - 31004
NISHIMURA, K. ET AL., J. BIOL. CHEM., vol. 286, 2011, pages 4760 - 4771
Attorney, Agent or Firm:
SHOBAYASHI, Masayuki (JP)
Right wood Masayuki (JP)
Right wood Masayuki (JP)
Download PDF: