Title:
METHOD FOR PRODUCING PERIPHERAL NERVE CELLS
Document Type and Number:
WIPO Patent Application WO/2018/074567
Kind Code:
A1
Abstract:
Provided is a method for efficiently producing peripheral nerve cells at high purity from undifferentiated cells. This method for producing peripheral nerve cells is for producing peripheral nerve cells from undifferentiated cells with the potency to differentiate into peripheral nerve cells, the method comprising the following steps: (a) culturing undifferentiated cells with the potency to differentiate into peripheral nerve cells and inducing differentiation into nerve progenitor cells without detaching grown colonies from a culture vessel; and (b) after detaching nerve progenitor cells generated in step (a) from the culture vessel, seeding the cells into the culture vessel at a seeding density of 2×105
to 6×105 cells/cm2 and culturing the cells for 14-42 days.
Inventors:
NISHIYAMA MASAHIKO (JP)
ROKUDAI SUSUMU (JP)
YOSHIYAMA SHINJI (JP)
TAKAHASHI HIROYUKI (JP)
ROKUDAI SUSUMU (JP)
YOSHIYAMA SHINJI (JP)
TAKAHASHI HIROYUKI (JP)
Application Number:
PCT/JP2017/037922
Publication Date:
April 26, 2018
Filing Date:
October 20, 2017
Export Citation:
Assignee:
UNIV GUNMA NAT UNIV CORP (JP)
YAKULT HONSHA KK (JP)
YAKULT HONSHA KK (JP)
International Classes:
C12N5/00; C12N5/0793; C12N5/0797; C12N5/10
Foreign References:
| JP2013532961A | 2013-08-22 |
Other References:
POMP, O. ET AL.: "Generation of peripheral sensory and sympathetic neurons and neural crest cells from human embryonic stem cells", STEM CELLS, vol. 23, 2005, pages 923 - 930, XP009054360
N AKAMURA, MAI ET AL.: "Differentiationpatterns of mouse embryonic stem cells and induced pluripotent stem cells into neurons", CYTOTECHNOLOGY, vol. 68, 10 April 2016 (2016-04-10), pages 409 - 417, XP035905663
LEE, K. S. ET AL.: "Human sensory neurons derived from induced pluripotent stem cells support varicella-zoster virus infection", PLOS ONE, vol. 7, no. 12, 2012, pages e53010, XP055366496
SHI, F. X. ET AL.: "BMP4 induction of sensory neurons from human embryonic stem cells and reinnervation of sensory epithelium", EUROPEAN JOUNAL OF NEUROSCIENCE, vol. 26, 2007, pages 3016 - 3023, XP055070150
GUO, X. F. ET AL.: "Derivation of sensory neurons and neural crest stem cells from human neural progenitor hNP1", BIOMATERIALS, vol. 34, no. 18, 2013, pages 4418 - 4427, XP028526273
GUNTHER, K. ET AL.: "Rapid monolayer neural induction of induced pluripotent stem cells yields stably proliferating neural stem cells", JOURNAL OF STEM CELL RESEARCH AND THERAPY, vol. 6, no. 6, 20 June 2016 (2016-06-20), pages 1 - 6, XP055486141
NAT , R.: "From human pluripotent stem cells to peripheral neurons, Pluripotent Stem Cells-From the Bench to the Clinic", INTECH, 20 July 2016 (2016-07-20), pages 307 - 329, XP055486145, Retrieved from the Internet [retrieved on 20180111]
See also references of EP 3530728A4
N AKAMURA, MAI ET AL.: "Differentiationpatterns of mouse embryonic stem cells and induced pluripotent stem cells into neurons", CYTOTECHNOLOGY, vol. 68, 10 April 2016 (2016-04-10), pages 409 - 417, XP035905663
LEE, K. S. ET AL.: "Human sensory neurons derived from induced pluripotent stem cells support varicella-zoster virus infection", PLOS ONE, vol. 7, no. 12, 2012, pages e53010, XP055366496
SHI, F. X. ET AL.: "BMP4 induction of sensory neurons from human embryonic stem cells and reinnervation of sensory epithelium", EUROPEAN JOUNAL OF NEUROSCIENCE, vol. 26, 2007, pages 3016 - 3023, XP055070150
GUO, X. F. ET AL.: "Derivation of sensory neurons and neural crest stem cells from human neural progenitor hNP1", BIOMATERIALS, vol. 34, no. 18, 2013, pages 4418 - 4427, XP028526273
GUNTHER, K. ET AL.: "Rapid monolayer neural induction of induced pluripotent stem cells yields stably proliferating neural stem cells", JOURNAL OF STEM CELL RESEARCH AND THERAPY, vol. 6, no. 6, 20 June 2016 (2016-06-20), pages 1 - 6, XP055486141
NAT , R.: "From human pluripotent stem cells to peripheral neurons, Pluripotent Stem Cells-From the Bench to the Clinic", INTECH, 20 July 2016 (2016-07-20), pages 307 - 329, XP055486145, Retrieved from the Internet
See also references of EP 3530728A4
Attorney, Agent or Firm:
THE PATENT CORPORATE BODY ARUGA PATENT OFFICE (JP)
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