Title:
METHOD FOR PRODUCING PHYTOSPHINGOSINE OR PHYTOCERAMIDE
Document Type and Number:
WIPO Patent Application WO/2022/158535
Kind Code:
A1
Abstract:
A method for producing an objective substance such as phytosphingosine (PHS) and phytoceramide (PHC) using yeast is provided. An objective substance is produced by cultivating yeast having an ability to produce the objective substance and modified so that the expression and/or activity of a protein(s) encoded by NEM1 and/or SPO7 gene(s) is reduced in a culture medium.
Inventors:
RIEZMAN HOWARD (CH)
TAVARES SABINA DE ANDRADE PEREIRA (CH)
SCHWAB MARKUS (CH)
SEREBRIANYI VSEVOLOD ALEKSANDROVICH (RU)
SOFYANOVICH OLGA ALEKSANDROVNA (RU)
OZEROVA ANNA MIKHAILOVNA (RU)
IWANAGA NAOKI (JP)
TAVARES SABINA DE ANDRADE PEREIRA (CH)
SCHWAB MARKUS (CH)
SEREBRIANYI VSEVOLOD ALEKSANDROVICH (RU)
SOFYANOVICH OLGA ALEKSANDROVNA (RU)
OZEROVA ANNA MIKHAILOVNA (RU)
IWANAGA NAOKI (JP)
Application Number:
PCT/JP2022/002034
Publication Date:
July 28, 2022
Filing Date:
January 20, 2022
Export Citation:
Assignee:
AJINOMOTO KK (JP)
EVOLVA SA (CH)
EVOLVA SA (CH)
International Classes:
C12P13/00; C12N9/16; C12P13/02
Domestic Patent References:
| WO2017033463A1 | 2017-03-02 | |||
| WO2017033463A1 | 2017-03-02 | |||
| WO2017033464A1 | 2017-03-02 | |||
| WO1995012683A1 | 1995-05-11 | |||
| WO1995032289A1 | 1995-11-30 |
Foreign References:
| JP2014529400A | 2014-11-13 | |||
| US5869711A | 1999-02-09 |
Other References:
SU, W.-M. ET AL.: "Yeast Nem1-Spo7 Protein Phosphatase Activity on Pah1 Phosphatidate Phosphatase Is Specific for the Pho85-Pho80 Protein Kinase Phosphorylation Sites", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 289, no. 50, 12 December 2014 (2014-12-12), pages 34699 - 34708, XP055932308, Retrieved from the Internet DOI: 10.1074/jbc.M114.614883
SU WM: "Yeast Neml-Spo7 protein phosphatase activity on Pahl phosphatidate phosphatase is specific for the Pho85-Pho80 protein kinase phosphorylation sites", J BIOL CHEM., vol. 289, no. 50, 12 December 2014 (2014-12-12), pages 34699 - 708
ACTA DERM VENEREOL, vol. 82, no. 3, 2002, pages 170 - 3
INT J SYST EVOL MICROBIOL, vol. 64, March 2014 (2014-03-01), pages 1057 - 61
SCHORSCH ET AL., CURR GENET, vol. 55, 2009, pages 381 - 9
PLANT CELL, vol. 18, no. 12, December 2006 (2006-12-01), pages 3576 - 93
J BIOL CHEM., vol. 275, no. 11, 17 March 2000 (2000-03-17), pages 7597 - 603
BIOCHIM BIOPHYS ACTA, vol. 1761, no. 1, January 2006 (2006-01-01), pages 52 - 63
GUILLAS, KIRCHMANCHUARD, PFEFFERLIJIANG, JAZWINSKICONZELMAN, EMBO J., vol. 20, 2001, pages 2655 - 2665
SCHORLING, VALLEEBARZ, REIZMANOESTERHELT, MOL. BIOL. CELL, vol. 12, 2001, pages 3417 - 3427
VALLEERIEZMAN, EMBO J., vol. 24, 2005, pages 730 - 741
J BIOL CHEM., vol. 275, no. 10, 10 March 2000 (2000-03-10), pages 6876 - 84
SU WM, J BIOL CHEM., vol. 289, no. 50, 12 December 2014 (2014-12-12), pages 34699 - 708
J BIOL CHEM., vol. 278, no. 9, 28 February 2003 (2003-02-28), pages 7325 - 34
PLANT PHYSIOL., vol. 137, no. 2, February 2005 (2005-02-01), pages 724 - 37
J BIOL CHEM., vol. 272, no. 28, 11 July 1997 (1997-07-11), pages 17376 - 84
EUKARYOT CELL, vol. 2, no. 2, April 2003 (2003-04-01), pages 284 - 94
GENE, vol. 192, no. 2, 19 June 1997 (1997-06-19), pages 245 - 50
EUR J BIOCHEM., vol. 123, no. 3, April 1982 (1982-04-01), pages 571 - 6
MILLER, J.H: "Experiments in Molecular Genetics", 1972, COLD SPRING HARBOR LABORATORY
"Genetic Engineering", vol. 8, 1987, KYORITSU SHUPPAN CO., LTD, article "Fundamental Microbiology"
GENE, vol. 60, no. 1, 1987, pages 115 - 127
NAKAMURA, Y. ET AL.: "Codon Usage Database", NUCL. ACIDS RES., vol. 28, 2000, pages 292, Retrieved from the Internet
H.ITO ET AL., J. BATERIOL., 1983, pages 153 - 163
FERMENTATION AND INDUSTRY, vol. 43, 1985, pages 630 - 637
LUIS ET AL., FEMS MICRO BIOLOGY LETTERS, vol. 165, 1998, pages 335 - 340
GIETZ R.D.SCHIESTL R.H., METHODS MOL.CELL. BIOL., vol. 5, 1995, pages 255 - 269
SAMBROOK, J. ET AL.: "Molecular Cloning A Laboratory Manual", 2001, COLD SPRING HARBOR LABORATORY PRESS
JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 272, 1997, pages 8611 - 8617
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, USA, vol. 95, 1998, pages 5511 - 5515
JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 26, no. 116, 1991, pages 20833 - 20839
SU WM: "Yeast Neml-Spo7 protein phosphatase activity on Pahl phosphatidate phosphatase is specific for the Pho85-Pho80 protein kinase phosphorylation sites", J BIOL CHEM., vol. 289, no. 50, 12 December 2014 (2014-12-12), pages 34699 - 708
ACTA DERM VENEREOL, vol. 82, no. 3, 2002, pages 170 - 3
INT J SYST EVOL MICROBIOL, vol. 64, March 2014 (2014-03-01), pages 1057 - 61
SCHORSCH ET AL., CURR GENET, vol. 55, 2009, pages 381 - 9
PLANT CELL, vol. 18, no. 12, December 2006 (2006-12-01), pages 3576 - 93
J BIOL CHEM., vol. 275, no. 11, 17 March 2000 (2000-03-17), pages 7597 - 603
BIOCHIM BIOPHYS ACTA, vol. 1761, no. 1, January 2006 (2006-01-01), pages 52 - 63
GUILLAS, KIRCHMANCHUARD, PFEFFERLIJIANG, JAZWINSKICONZELMAN, EMBO J., vol. 20, 2001, pages 2655 - 2665
SCHORLING, VALLEEBARZ, REIZMANOESTERHELT, MOL. BIOL. CELL, vol. 12, 2001, pages 3417 - 3427
VALLEERIEZMAN, EMBO J., vol. 24, 2005, pages 730 - 741
J BIOL CHEM., vol. 275, no. 10, 10 March 2000 (2000-03-10), pages 6876 - 84
SU WM, J BIOL CHEM., vol. 289, no. 50, 12 December 2014 (2014-12-12), pages 34699 - 708
J BIOL CHEM., vol. 278, no. 9, 28 February 2003 (2003-02-28), pages 7325 - 34
PLANT PHYSIOL., vol. 137, no. 2, February 2005 (2005-02-01), pages 724 - 37
J BIOL CHEM., vol. 272, no. 28, 11 July 1997 (1997-07-11), pages 17376 - 84
EUKARYOT CELL, vol. 2, no. 2, April 2003 (2003-04-01), pages 284 - 94
GENE, vol. 192, no. 2, 19 June 1997 (1997-06-19), pages 245 - 50
EUR J BIOCHEM., vol. 123, no. 3, April 1982 (1982-04-01), pages 571 - 6
MILLER, J.H: "Experiments in Molecular Genetics", 1972, COLD SPRING HARBOR LABORATORY
"Genetic Engineering", vol. 8, 1987, KYORITSU SHUPPAN CO., LTD, article "Fundamental Microbiology"
GENE, vol. 60, no. 1, 1987, pages 115 - 127
NAKAMURA, Y. ET AL.: "Codon Usage Database", NUCL. ACIDS RES., vol. 28, 2000, pages 292, Retrieved from the Internet
H.ITO ET AL., J. BATERIOL., 1983, pages 153 - 163
FERMENTATION AND INDUSTRY, vol. 43, 1985, pages 630 - 637
LUIS ET AL., FEMS MICRO BIOLOGY LETTERS, vol. 165, 1998, pages 335 - 340
GIETZ R.D.SCHIESTL R.H., METHODS MOL.CELL. BIOL., vol. 5, 1995, pages 255 - 269
SAMBROOK, J. ET AL.: "Molecular Cloning A Laboratory Manual", 2001, COLD SPRING HARBOR LABORATORY PRESS
JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 272, 1997, pages 8611 - 8617
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, USA, vol. 95, 1998, pages 5511 - 5515
JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 26, no. 116, 1991, pages 20833 - 20839
Attorney, Agent or Firm:
IP FIRM SHUWA (JP)
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