Title:
METHOD FOR PRODUCING PLASMID DNA USING ESCHERICHIA COLI
Document Type and Number:
WIPO Patent Application WO/2023/286629
Kind Code:
A1
Abstract:
The purpose of the present invention is to collect plasmid DNA from a cell body of Escherichia coli with high efficiency in the production of the plasmid DNA using the Escherichia coli. Provided is a method for producing plasmid DNA, the method comprising the following steps (a) to (c): (a) preparing Escherichia coli that has a mutation in a gene region involved in the retention of the properties of an outer membrane thereof and carries a desired plasmid; (b) culturing the Escherichia coli prepared in the step (a); and (c) collecting the desired plasmid from the cultured cells.
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Inventors:
TOKUNAGA TOMOHISA (JP)
NISHIYAMA TOZO (JP)
MINAKUCHI KAZUNOBU (JP)
CHO SEUNG HYUN (BE)
COLLET JEAN-FRANCOIS (BE)
DEGHELT MICHAEL (BE)
NISHIYAMA TOZO (JP)
MINAKUCHI KAZUNOBU (JP)
CHO SEUNG HYUN (BE)
COLLET JEAN-FRANCOIS (BE)
DEGHELT MICHAEL (BE)
Application Number:
PCT/JP2022/026275
Publication Date:
January 19, 2023
Filing Date:
June 30, 2022
Export Citation:
Assignee:
KANEKA CORP (JP)
KANEKA EUROGENTEC S A (BE)
UNIV CATHOLIQUE LOUVAIN (BE)
KANEKA EUROGENTEC S A (BE)
UNIV CATHOLIQUE LOUVAIN (BE)
International Classes:
C12P19/34; C12N1/21; C12N15/31; C12N15/52; C12N15/57
Domestic Patent References:
| WO2020020438A1 | 2020-01-30 | |||
| WO2020007493A1 | 2020-01-09 | |||
| WO1998014600A1 | 1998-04-09 |
Foreign References:
| CN110878276A | 2020-03-13 | |||
| JPH09509313A | 1997-09-22 | |||
| JP2021117545A | 2021-08-10 |
Other References:
GAO WEN, YIN JUN, BAO LICHEN, WANG QUN, HOU SHAN, YUE YALI, YAO WENBING, GAO XIANGDONG: "Engineering Extracellular Expression Systems in Escherichia coli Based on Transcriptome Analysis and Cell Growth State", ACS SYNTHETIC BIOLOGY, AMERICAN CHEMICAL SOCIETY, WASHINGTON DC ,USA, vol. 7, no. 5, 18 May 2018 (2018-05-18), Washington DC ,USA , pages 1291 - 1302, XP055794672, ISSN: 2161-5063, DOI: 10.1021/acssynbio.7b00400
SANDERS AKEISHA N., PAVELKA MARTIN S.: "Phenotypic analysis of Eschericia coli mutants lacking l,d-transpeptidases", MICROBIOLOGY, SOCIETY FOR GENERAL MICROBIOLOGY, READING, vol. 159, no. Pt_9, 1 September 2013 (2013-09-01), Reading , pages 1842 - 1852, XP093023960, ISSN: 1350-0872, DOI: 10.1099/mic.0.069211-0
H. C. BIMNOIMJ. DOLY, NUCLEIC ACIDS RES., vol. 7, no. 6, 1979, pages 1513 - 1523
"GenBank", Database accession no. CAD5993943.1
"NCBI", Database accession no. NP_418559.1
FEMS MICROBIOLOGY LETTERS, vol. 165, 1998, pages 335 - 340
JOURNAL OF BACTERIOLOGY, December 1995 (1995-12-01), pages 7171 - 7177
CURR GENET, vol. 10, no. 8, 1986, pages 573 - 578
DATSENKO K.AWANNER B.L, PROC. NATL. ACAD. SCI., U.S.A., vol. 97, no. 12, 2000, pages 6640 - 6645
LINK A. J. ET AL., J. BACTERIOL, vol. 179, 1997, pages 6228 - 6237
CASCALES ELLOUBES R, MOL. MICROBIOL, vol. 51, no. 3, 2004, pages 873 - 885
M. BRAUNT. J. SILHAVY, MOL MICROBIOL, vol. 45, no. 5, 2002, pages 1289 - 302
THOMASON ET AL., PLASMID, vol. 58, no. 2, September 2007 (2007-09-01), pages 148 - 58
BABA ET AL., MOL SYST BIOL, vol. 2, 2006, pages 2006
CURR PROTOC MOL BIOL, July 2007 (2007-07-01)
SANDERS AKEISHA N., PAVELKA MARTIN S.: "Phenotypic analysis of Eschericia coli mutants lacking l,d-transpeptidases", MICROBIOLOGY, SOCIETY FOR GENERAL MICROBIOLOGY, READING, vol. 159, no. Pt_9, 1 September 2013 (2013-09-01), Reading , pages 1842 - 1852, XP093023960, ISSN: 1350-0872, DOI: 10.1099/mic.0.069211-0
H. C. BIMNOIMJ. DOLY, NUCLEIC ACIDS RES., vol. 7, no. 6, 1979, pages 1513 - 1523
"GenBank", Database accession no. CAD5993943.1
"NCBI", Database accession no. NP_418559.1
FEMS MICROBIOLOGY LETTERS, vol. 165, 1998, pages 335 - 340
JOURNAL OF BACTERIOLOGY, December 1995 (1995-12-01), pages 7171 - 7177
CURR GENET, vol. 10, no. 8, 1986, pages 573 - 578
DATSENKO K.AWANNER B.L, PROC. NATL. ACAD. SCI., U.S.A., vol. 97, no. 12, 2000, pages 6640 - 6645
LINK A. J. ET AL., J. BACTERIOL, vol. 179, 1997, pages 6228 - 6237
CASCALES ELLOUBES R, MOL. MICROBIOL, vol. 51, no. 3, 2004, pages 873 - 885
M. BRAUNT. J. SILHAVY, MOL MICROBIOL, vol. 45, no. 5, 2002, pages 1289 - 302
THOMASON ET AL., PLASMID, vol. 58, no. 2, September 2007 (2007-09-01), pages 148 - 58
BABA ET AL., MOL SYST BIOL, vol. 2, 2006, pages 2006
CURR PROTOC MOL BIOL, July 2007 (2007-07-01)
Attorney, Agent or Firm:
HIRAKI & ASSOCIATES (JP)
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