Title:
METHOD FOR PRODUCING A PLURALITY OF TEETH FROM ONE ISOLATED TOOTH GERM
Document Type and Number:
WIPO Patent Application WO/2014/126007
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a method for producing a plurality of teeth from one isolated tooth germ in order to increase the overall number of tooth germs which can be used for transplantation. The present invention relates to a method for producing teeth. More specifically, the present invention provides a method for producing a plurality of teeth from one isolated tooth germ including an epithelial-tissue layer and a mesenchymal-tissue layer, said method being characterized by including: (a) a step in which the one isolated tooth germ including the epithelial-tissue layer and the mesenchymal-tissue layer is entirely or partially divided, said division being characterized in that each divided tooth-germ part is divided so as to include a portion of the epithelial-tissue layer and a portion of the mesenchymal-tissue layer; and (b) a step in which a plurality of teeth are formed by either culturing the divided tooth germ in vitro, or culturing the divided tooth germ in vivo in a non-human animal.
Inventors:
TSUJI TAKASHI (JP)
OSHIMA MASAMITSU (JP)
OSHIMA MASAMITSU (JP)
Application Number:
PCT/JP2014/052858
Publication Date:
August 21, 2014
Filing Date:
February 07, 2014
Export Citation:
Assignee:
ORGAN TECHNOLOGIES INC (JP)
International Classes:
C12N5/073; A61K6/02; A61L27/00
Domestic Patent References:
| WO2006129672A1 | 2006-12-07 |
Other References:
OSHIMA,M. ET AL.: "Generation of a Bioengineered Tooth by Using a Three-Dimensional Cell Manipulation Method (Organ Germ Method).", METHODS MOL.BIOL., vol. 887, 2012, pages 149 - 165, XP008181073
OSHIMA,M. ET AL.: "Functional Tooth Regeneration Using a Bioengineered Tooth Unit as a Mature Organ Replacement Regenerative Therapy.", PLOS ONE, vol. 6, no. 7, 2011, pages E21531, XP055292823
MASAMITSU OSHIMA ET AL.: "Jisedai no Shika Saisei Chiryo no Jitsugen ni Mukete", DENTAL OUTLOOK, vol. 118, no. 5, 2011, pages 774 - 778, XP008181085
NATURE METHODS, vol. 4, 2007, pages 227 - 230
PNAS, vol. 11, no. 106, 2009, pages 13475 - 80
OSHIMA,M. ET AL.: "Functional Tooth Regeneration Using a Bioengineered Tooth Unit as a Mature Organ Replacement Regenerative Therapy.", PLOS ONE, vol. 6, no. 7, 2011, pages E21531, XP055292823
MASAMITSU OSHIMA ET AL.: "Jisedai no Shika Saisei Chiryo no Jitsugen ni Mukete", DENTAL OUTLOOK, vol. 118, no. 5, 2011, pages 774 - 778, XP008181085
NATURE METHODS, vol. 4, 2007, pages 227 - 230
PNAS, vol. 11, no. 106, 2009, pages 13475 - 80
Attorney, Agent or Firm:
NANJO, Masahiro et al. (JP)
Masahiro Nanjo (JP)
Masahiro Nanjo (JP)
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