Title:
METHOD FOR PRODUCING PLURIPOTENT STEM CELLS
Document Type and Number:
WIPO Patent Application WO/2023/054357
Kind Code:
A1
Abstract:
Provided is a method for producing pluripotent stem cells from somatic cells, the method comprising: (1) a step of seeding somatic cells into a container having one or more sections in which two or more somatic cells can be gathered; (2) a step of bringing the somatic cells into contact with an initializing factor; and (3) a step of culturing, in a state in which the two or more somatic cells have been gathered in the sections, the somatic cells having been contacted with the initializing factor.
Inventors:
TSUKAHARA MASAYOSHI (JP)
YAMAMOTO YUKO (JP)
YAMAMOTO YUKO (JP)
Application Number:
PCT/JP2022/035937
Publication Date:
April 06, 2023
Filing Date:
September 27, 2022
Export Citation:
Assignee:
CIRA FOUND (JP)
International Classes:
C12N5/10; C12M1/00
Domestic Patent References:
| WO2008106771A1 | 2008-09-12 | |||
| WO2017115865A1 | 2017-07-06 | |||
| WO2018155595A1 | 2018-08-30 | |||
| WO2017038887A1 | 2017-03-09 |
Foreign References:
| JP2013070636A | 2013-04-22 | |||
| JP2020022480A | 2020-02-13 |
Other References:
YE HUAHU, WANG QIWEI: "Efficient Generation of Non-Integration and Feeder-Free Induced Pluripotent Stem Cells from Human Peripheral Blood Cells by Sendai Virus", CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, vol. 50, no. 4, 1 January 2018 (2018-01-01), CH , pages 1318 - 1331, XP093052870, ISSN: 1015-8987, DOI: 10.1159/000494589
KANIE KEI, SAKAI TEPPEI, IMAI YUTA, YOSHIDA KEI, SUGIMOTO AYAKO, MAKINO HODAKA, KUBO HIROTSUGU, KATO RYUJI: "Effect of mechanical vibration stress in cell culture on human induced pluripotent stem cells", REGENERATIVE THERAPY, vol. 12, 1 December 2019 (2019-12-01), pages 27 - 35, XP093052873, ISSN: 2352-3204, DOI: 10.1016/j.reth.2019.05.002
KANIE KEI, SAKAI TEPPEI, IMAI YUTA, YOSHIDA KEI, SUGIMOTO AYAKO, MAKINO HODAKA, KUBO HIROTSUGU, KATO RYUJI: "Effect of mechanical vibration stress in cell culture on human induced pluripotent stem cells", REGENERATIVE THERAPY, vol. 12, 1 December 2019 (2019-12-01), pages 27 - 35, XP093052873, ISSN: 2352-3204, DOI: 10.1016/j.reth.2019.05.002
Attorney, Agent or Firm:
YAMAO, Norihito et al. (JP)
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