Title:
METHOD FOR PRODUCING POLYHYDROXYALKANOIC ACID, AND MICROBES
Document Type and Number:
WIPO Patent Application WO/2018/117168
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide: a method for producing polyhydroxyalkanoic acid (PHA) with microbes, the method having an improved PHA productivity; and PHA-producing microbes that are used for said method of production. Provided is a method for producing polyhydroxyalkanoic acid, comprising a step for culturing microbes that have a polyhydroxyalkanoic acid synthase gene and have an inactivated gene that codes for a flagellar protein, and causing the microbes to produce polyhydroxyalkanoic acid. The microbes may be ones for which a lipase, a phosphatase, or a protein shown by the amino acid sequences listed in SEQ ID NO: 6 or 7 has been inactivated. The microbes may be Cupriavidus necator.
Inventors:
SATO SHUNSUKE
ARIKAWA HISASHI
AOKI RINA
ARIKAWA HISASHI
AOKI RINA
Application Number:
PCT/JP2017/045772
Publication Date:
June 28, 2018
Filing Date:
December 20, 2017
Export Citation:
Assignee:
KANEKA CORP (JP)
International Classes:
C12P7/62; C12N1/21
Domestic Patent References:
| WO2002097089A1 | 2002-12-05 | |||
| WO2015146195A1 | 2015-10-01 |
Foreign References:
| JP2007259708A | 2007-10-11 |
Other References:
ZHENG, Q. ET AL.: "Paracoccus beibuensis sp. nov., Isolated from the South China Sea", CURR MICROBIOL, vol. 62, no. 3, 2011, pages 710 - 714, XP019886185
BURDMAN, S. ET AL.: "Aggregation in Azospirillum brasilense: effects of chemical and physical factors and involvement of extracellular components", MICROBIOLOGY, vol. 144, no. 7, 1998, pages 1989 - 1999, XP055494810
DATABASE UniProtKB [o] 3 October 2006 (2006-10-03), Database accession no. QOK217
DATABASE UniProtKB [o] 3 October 2006 (2006-10-03), Database accession no. QOK868
DATABASE UniProtKB [o] 3 October 2006 (2006-10-03), Database accession no. QOK9P1
DATABASE UniProtKB [o] 3 October 2006 (2006-10-03), Database accession no. QOK9P2
DATABASE UniProtKB [o] 3 October 2006 (2006-10-03), Database accession no. QOK5D2
DATABASE UniProtKB [o] 3 October 2006 (2006-10-03), Database accession no. QOK215
FUJIKI, TETSUYA: "Biodegradable plastic production by microorganism", MICROBIOL. CULT. COLL., vol. 29, no. 1, 2013, pages 25 - 29, XP009515346, ISSN: 1342-4041
ANDERSONAJ. ET AL., HIT. J. BIOL. MACROMOL., vol. 12, 1990, pages 201 - 105
SATO S. ET AL., J. BIOSCI. BIOENG., vol. 120, no. 3, 2015, pages 246 - 251
INSOMPHUN C. ET AL., METAB. ENG., vol. 27, 2015, pages 38 - 45
RABERG M. ET AL., APP. ENVIRON. MICROBIOL., vol. 74, no. 14, 2008, pages 4477 - 4490
LOCHER KP. ET AL., PHIL. TRANS. R. SOC. B, vol. 346, 2009, pages 239 - 245
See also references of EP 3561065A4
BURDMAN, S. ET AL.: "Aggregation in Azospirillum brasilense: effects of chemical and physical factors and involvement of extracellular components", MICROBIOLOGY, vol. 144, no. 7, 1998, pages 1989 - 1999, XP055494810
DATABASE UniProtKB [o] 3 October 2006 (2006-10-03), Database accession no. QOK217
DATABASE UniProtKB [o] 3 October 2006 (2006-10-03), Database accession no. QOK868
DATABASE UniProtKB [o] 3 October 2006 (2006-10-03), Database accession no. QOK9P1
DATABASE UniProtKB [o] 3 October 2006 (2006-10-03), Database accession no. QOK9P2
DATABASE UniProtKB [o] 3 October 2006 (2006-10-03), Database accession no. QOK5D2
DATABASE UniProtKB [o] 3 October 2006 (2006-10-03), Database accession no. QOK215
FUJIKI, TETSUYA: "Biodegradable plastic production by microorganism", MICROBIOL. CULT. COLL., vol. 29, no. 1, 2013, pages 25 - 29, XP009515346, ISSN: 1342-4041
ANDERSONAJ. ET AL., HIT. J. BIOL. MACROMOL., vol. 12, 1990, pages 201 - 105
SATO S. ET AL., J. BIOSCI. BIOENG., vol. 120, no. 3, 2015, pages 246 - 251
INSOMPHUN C. ET AL., METAB. ENG., vol. 27, 2015, pages 38 - 45
RABERG M. ET AL., APP. ENVIRON. MICROBIOL., vol. 74, no. 14, 2008, pages 4477 - 4490
LOCHER KP. ET AL., PHIL. TRANS. R. SOC. B, vol. 346, 2009, pages 239 - 245
See also references of EP 3561065A4
Attorney, Agent or Firm:
PATENT CORPORATE BODY ARCO PATENT OFFICE (JP)
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