METHOD FOR PRODUCING POLYPEPTIDE, TAG, EXPRESSION VECTOR, METHOD FOR EVALUATING POLYPEPTIDE, METHOD FOR PRODUCING NUCLEIC ACID DISPLAY LIBRARY, AND SCREENING METHOD

Title:

METHOD FOR PRODUCING POLYPEPTIDE, TAG, EXPRESSION VECTOR, METHOD FOR EVALUATING POLYPEPTIDE, METHOD FOR PRODUCING NUCLEIC ACID DISPLAY LIBRARY, AND SCREENING METHOD

Document Type and Number:

WIPO Patent Application WO/2023/048290

Kind Code:

A1

Abstract:

This method for producing a polypeptide comprising expressing a polypeptide as a tag-added polypeptide comprises expressing the tag-added polypeptide from a nucleic acid having: a base sequence which is arranged immediately after a start codon and encodes a tag including the amino acid sequence of SEQ ID NO: 1; and a base sequence which is arranged in a frame downstream from the base sequence encoding the tag and encodes the polypeptide. SEQ ID NO: 1: Val-Lys-Lys-(Xaa)n. (Xaa)n is a sequence of n of any amino acids, wherein n is an integer of 1-8, and amino acids constituting (Xaa)n may be at least one kind or at least two kinds.

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Inventors:

MATSUI KEN (JP)
TOMINAGA JO (JP)
KIHARA SHIORI (JP)
HOSAKA TAKAHIRO (JP)
WATANABE TAKAYOSHI (JP)

Application Number:

PCT/JP2022/035787

Publication Date:

March 30, 2023

Filing Date:

September 26, 2022

Export Citation:

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Assignee:

FUJIFILM CORP (JP)

International Classes:

C12N15/67; C07K5/103; C07K7/06; C07K19/00; C12N15/63; C12P21/02; C12P21/04; C40B40/08; C40B40/10

Domestic Patent References:

WO2016204198A1	2016-12-22
WO2015030014A1	2015-03-05
WO2016204198A1	2016-12-22

Foreign References:

US8734812B1	2014-05-27
JP2021157186A	2021-10-07

Other References:

GULYAEVA N., ZASLAVSKY A., CHAIT A., ZASLAVSKY B.: "Relative hydrophobicity of di- to hexapeptides as measured by aqueous two-phase partitioning : Hydrophobicity of di- to hexapeptides", JOURNAL OF PEPTIDE RESEARCH, BLACKWELL PUBLISHING LTD., OXFORD; GB, vol. 61, no. 3, 1 March 2003 (2003-03-01), OXFORD; GB , pages 129 - 139, XP093054250, ISSN: 1397-002X, DOI: 10.1034/j.1399-3011.2003.00040.x
PROTEIN EXPRESSION AND PURIFICATION, vol. 70, 2010, pages 224 - 230
SCIENCE, vol. 342, 2013, pages 475 - 479
JOURNAL OF SYNTHETIC ORGANIC CHEMISTRY, JAPAN, vol. 75, no. 11, 2017, pages 1171 - 1178

Attorney, Agent or Firm:

TAIYO, NAKAJIMA & KATO (JP)

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