METHOD FOR PRODUCING POLYPHENOL-CONTAINING COMPOSITION

WIPO Patent Application WO/2022/210735

A1

The present invention provides a technical means for efficiently producing a polyphenol-containing composition from grass-based biomass. More specifically, the present invention provides a method for producing a polyphenol-containing composition derived from grass-based biomass, the method including: (1) a step in which an extraction liquid is obtained by bringing grass-based biomass into contact with an alkaline aqueous solution of 60°C or higher; (2) a step in which the extraction liquid obtained in step (1) is cooled to 60°C or lower to obtain a cooled extract solution; (3) a step in which the pH of the cooled extraction liquid obtained in step (2) is adjusted to a pH of 3.2-4.5, and the cooled extraction liquid is reacted with an enzyme having cellobiohydrolase activity and xylanase activity to obtain an enzyme-reacted solution; (4) a step in which a clarified liquid is obtained by rough filtration of the enzyme-reacted solution obtained in step (3); (5) a step in which the clarified liquid obtained in step (4) is passed through a column filled with an aromatic synthetic adsorbent composed of an aromatic resin subjected to a special treatment for increasing the specific surface area thereof, and the component adsorbed to the aromatic synthetic adsorbent is eluted using a mixed solvent of ethanol and water so as to obtain an eluted fraction as a polyphenol-containing composition.

ASAHI YUKA (JP)
MURAKAMI NATSUKO (JP)
HATTA CHIHARU (JP)
ZOUTA MOTOMI (JP)
NAKAJIMA HISANORI (JP)
MINAMINO ATSUSHI (TH)
ITOU SATORU (TH)

PCT/JP2022/015549

October 06, 2022

March 29, 2022

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MITSUI SUGAR CO (JP)
CELLULOSIC BIOMASS TECH CO LTD (TH)

C12P7/22; C07G1/00

WO2019230803A1	2019-12-05
WO2013018678A1	2013-02-07

NAKAMURA Yukitaka et al. (JP)

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