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Title:
METHOD FOR PRODUCING PROTEIN INCLUDING Κ CHAIN VARIABLE REGION
Document Type and Number:
WIPO Patent Application WO/2017/191747
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a method for efficiently producing a protein including a κ chain variable region using a specific κ chain variable region-binding peptide having high alkali tolerance as a ligand, and without requiring frequent replacement of the affinity separation matrix. This method for producing a κ chain variable region-containing protein is characterized by comprising a first step for bringing a liquid sample including a κ chain variable region-containing protein into contact with an affinity separation matrix on which a specific κ chain variable region-binding peptide has been immobilized as a ligand, a second step for washing the affinity separation matrix, a third step for separating the κ chain variable region-containing protein, and a fourth step for regenerating the affinity separation matrix, wherein the first to third steps are repeated at least three times.

Inventors:
YOSHIDA, Shinichi (1-8 Takasagocho Miyamaecho, Takasago-sh, Hyogo 88, 〒6768688, JP)
MURATA, Dai (1-8 Takasagocho Miyamaecho, Takasago-sh, Hyogo 88, 〒6768688, JP)
Application Number:
JP2017/015500
Publication Date:
November 09, 2017
Filing Date:
April 17, 2017
Export Citation:
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Assignee:
KANEKA CORPORATION (2-3-18, Nakanoshima Kita-ku, Osaka-sh, Osaka 88, 〒5308288, JP)
International Classes:
C07K1/22; C07K16/00; C12N15/00; C12N15/09
Domestic Patent References:
WO2015041218A12015-03-26
WO2016121701A12016-08-04
Foreign References:
CN1634990A2005-07-06
JPH07506573A1995-07-20
Other References:
PALMGREN, RONNIE ET AL.: "Improving the alkali stability of the kappa light chain-binding polypeptide from domain of peptostreptococcus protein L, Abstracts of Papers", 251ST ACS NATIONAL MEETING & EXPOSITION, 13 March 2016 (2016-03-13)
Attorney, Agent or Firm:
UEKI, Kyuichi et al. (Fujita-Toyobo Building 9th floor, 1-16 Dojima 2-chome, Kita-ku, Osaka-sh, Osaka 03, 〒5300003, JP)
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