Title:
METHOD OF PRODUCING PROTEIN
Document Type and Number:
WIPO Patent Application WO/2004/050891
Kind Code:
A1
Abstract:
It is intended to provide a method of synthesizing a protein at a high synthesis efficiency by which a translation reaction can be continued for a long time in a cell-free protein synthesis system, which is applicable to a large-scaled reaction system and which can be carried out by using a simple apparatus. It is also intended to provide a method of performing the synthesis and purification of a protein in a single container after the completion of the synthesis reaction as described above. Moreover, it is intended to provide a reagent kit and an apparatus for carrying out these methods. Namely, a method of producing a target protein with the use of a cell-free protein synthesis system characterized in that the protein is synthesized by bringing a liquid mixture for a transcription/translation reaction or a translation reaction into contact, via an interface, with a gel or a sol containing a substance to be consumed in the transcription/translation reaction or the translation reaction in both of its matrix and its exclusion volume.
Inventors:
MATSUBARA KEIKO (JP)
Application Number:
PCT/JP2003/015593
Publication Date:
June 17, 2004
Filing Date:
December 05, 2003
Export Citation:
Assignee:
ZOEGENE CORP (JP)
MATSUBARA KEIKO (JP)
MATSUBARA KEIKO (JP)
International Classes:
C12P21/02; (IPC1-7): C12P21/02; C12M1/00
Domestic Patent References:
| WO2002024939A1 | 2002-03-28 | |||
| WO2000068412A1 | 2000-11-16 | |||
| WO2001002548A2 | 2001-01-11 |
Foreign References:
| JP2002162353A | 2002-06-07 |
Other References:
YAMAGUCHI T.: "Ippan Seitbutsugaku", 1ST EDITION, KABUSHIKI KAISHA GIHODO, 10 September 1968 (1968-09-10), pages 12 - 13, XP002977665
OGINO K. ET AL: "Gel-soft Material no Kiso to Oyo-", 1STE EDITION, SANGYO TOSHO KABUSHIKI KAISHA, 20 May 1991 (1991-05-20), pages 153 - 160, XP002977666
ALEXANDER S. ET AL: "A Continuous cell-free translation system capable of producing polypeptides in high yield", SCIENCE, vol. 242, 1988, pages 1162 - 1164, XP002044225
ISHIKAWA T.ET AL.: "Zukai Biseibutsugaku Handbook", 1ST EDITION, MARUZEN CO., LTS, 30 September 1990 (1990-09-30), pages 42 - 47, XP002977668
OGINO K. ET AL: "Gel-soft Material no Kiso to Oyo-", 1STE EDITION, SANGYO TOSHO KABUSHIKI KAISHA, 20 May 1991 (1991-05-20), pages 153 - 160, XP002977666
ALEXANDER S. ET AL: "A Continuous cell-free translation system capable of producing polypeptides in high yield", SCIENCE, vol. 242, 1988, pages 1162 - 1164, XP002044225
ISHIKAWA T.ET AL.: "Zukai Biseibutsugaku Handbook", 1ST EDITION, MARUZEN CO., LTS, 30 September 1990 (1990-09-30), pages 42 - 47, XP002977668
Attorney, Agent or Firm:
SIKS & CO. (Kyobashi-Nisshoku Bldg. 8-7, Kyobashi 1-chom, Chuo-ku Tokyo, JP)
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