Title:
METHOD FOR PRODUCING RETINAL PIGMENT EPITHELIAL CELLS
Document Type and Number:
WIPO Patent Application WO/2015/068505
Kind Code:
A1
Abstract:
The present invention provides a method for producing retinal pigment epithelial cells, the method including: (1) a first step for forming an aggregate of pluripotent stem cells by suspension-culturing pluripotent stem cells in a serum-free medium; (2) a second step for suspension-culturing the aggregate formed in step (1) in a serum-free medium or serum medium including a BMP signal transduction pathway agonist and not including a sonic hedgehog signal transduction pathway agonist, and obtaining an aggregate including retinal precursor cells; and (3) a third step for suspension-culturing the aggregate obtained in step (2) in a serum-free medium or serum medium including a Wnt signal pathway agonist and not including a sonic hedgehog signal transduction pathway agonist or a BMP signal transduction pathway agonist, and obtaining an aggregate including retinal pigment epithelial cells.
Inventors:
NAKANO TOKUSHIGE (JP)
OZONE CHIKAFUMI (JP)
SASAI, YOSHIKI (笹井 芳樹)
OZONE CHIKAFUMI (JP)
SASAI, YOSHIKI (笹井 芳樹)
Application Number:
PCT/JP2014/076471
Publication Date:
May 14, 2015
Filing Date:
October 02, 2014
Export Citation:
Assignee:
SUMITOMO CHEMICAL CO (JP)
RIKEN (JP)
RIKEN (JP)
International Classes:
C12N5/0735; A61K35/12; A61K35/44; A61L27/00; A61P27/00; C12N5/074; C12N5/10
Domestic Patent References:
| WO2012173207A1 | 2012-12-20 | |||
| WO2013065763A1 | 2013-05-10 | |||
| WO2013077425A1 | 2013-05-30 | |||
| WO1998030679A1 | 1998-07-16 |
Foreign References:
| JP2013128474A | 2013-07-04 | |||
| JP2010524457A | 2010-07-22 |
Other References:
CELL STEM CELL, vol. 5, 2009, pages 396 - 408
CELL STEM CELL, vol. 10, no. 6, 2012, pages 771 - 785
CELL, vol. 131, no. 5, 2007, pages 861 - 872
CELL, vol. 126, no. 4, 2006, pages 663 - 676
YAMANAKA ET AL., CELL, vol. 126, no. 4, 2006, pages 663 - 676
SCIENCE, vol. 318, no. 5858, 2007, pages 1917 - 1920
NAT. BIOTECHNOL., vol. 26, no. 1, 2008, pages 101 - 106
"Manipulating the Mouse Embryo, A Laboratory Manual, second edition", 1994, COLD SPRING HARBOR LABORATORY PRESS
"Gene Targeting, A Practical Approach", 1993, IRL PRESS AT OXFORD UNIVERSITY PRESS
"Bio Manual series 8, gene targeting, Production of mutant mouse using ES cells", 1995, YODOSHA CO., LTD.
"Molecular Cloning, A Laboratory Manual, second edition", 1989, COLD SPRING HARBOR LABORATORY PRESS
"Current Protocols in Molecular Biology", 1987, JOHN WILEY & SONS
J. BIOL. CHEM., vol. 284, 2009, pages 7820 - 7831
UENO, M. ET AL., PNAS, vol. 103, no. 25, 2006, pages 9554 - 9559
WATANABE, K. ET AL., NAT BIOTECH, vol. 25, 2007, pages 681 - 686
See also references of EP 3070162A4
CELL STEM CELL, vol. 10, no. 6, 2012, pages 771 - 785
CELL, vol. 131, no. 5, 2007, pages 861 - 872
CELL, vol. 126, no. 4, 2006, pages 663 - 676
YAMANAKA ET AL., CELL, vol. 126, no. 4, 2006, pages 663 - 676
SCIENCE, vol. 318, no. 5858, 2007, pages 1917 - 1920
NAT. BIOTECHNOL., vol. 26, no. 1, 2008, pages 101 - 106
"Manipulating the Mouse Embryo, A Laboratory Manual, second edition", 1994, COLD SPRING HARBOR LABORATORY PRESS
"Gene Targeting, A Practical Approach", 1993, IRL PRESS AT OXFORD UNIVERSITY PRESS
"Bio Manual series 8, gene targeting, Production of mutant mouse using ES cells", 1995, YODOSHA CO., LTD.
"Molecular Cloning, A Laboratory Manual, second edition", 1989, COLD SPRING HARBOR LABORATORY PRESS
"Current Protocols in Molecular Biology", 1987, JOHN WILEY & SONS
J. BIOL. CHEM., vol. 284, 2009, pages 7820 - 7831
UENO, M. ET AL., PNAS, vol. 103, no. 25, 2006, pages 9554 - 9559
WATANABE, K. ET AL., NAT BIOTECH, vol. 25, 2007, pages 681 - 686
See also references of EP 3070162A4
Attorney, Agent or Firm:
TAKASHIMA, HAJIME (JP)
Takashima 1 (JP)
Takashima 1 (JP)
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