Title:
METHOD OF PRODUCING SAMPLE FOR GENE ANALYSIS
Document Type and Number:
WIPO Patent Application WO/2022/138873
Kind Code:
A1
Abstract:
[Problem] To provide a method of producing a sample for gene analysis for PCR, and a PCR method and a gene analysis method using the sample. [Solution] A method of producing a sample for gene analysis, said method comprising a collected substance suspension step for suspending a collected substance, which is cells or a tissue collected from a subject, in nuclease-free water.
Inventors:
MIZUKAMI YUSUKE (JP)
ONO YUSUKE (JP)
HAYASHI AKIHIRO (JP)
ONO YUSUKE (JP)
HAYASHI AKIHIRO (JP)
Application Number:
PCT/JP2021/048040
Publication Date:
June 30, 2022
Filing Date:
December 23, 2021
Export Citation:
Assignee:
NATIONAL UNIV CORPORATION ASAHIKAWA MEDICAL UNIV (JP)
TOKUSHUKAI MEDICAL CORP SAPPORO HIGASHI TOKUSHUKAI HOSPITAL (JP)
GENOMEDIA INC (JP)
TOKUSHUKAI MEDICAL CORP SAPPORO HIGASHI TOKUSHUKAI HOSPITAL (JP)
GENOMEDIA INC (JP)
International Classes:
C12Q1/24; C12Q1/686; G01N1/28; G01N33/53
Domestic Patent References:
| WO2019200342A1 | 2019-10-17 |
Foreign References:
| JP2015061544A | 2015-04-02 | |||
| JPH05192199A | 1993-08-03 | |||
| JPH05184398A | 1993-07-27 |
Other References:
LIN, MING-HONG ET AL.: "O-3-1 Development of Novel Diagnostic Method for Pancreatic Cancer by Digital PCR Using FNB Samples", JOURNAL OF THE JAPAN PANCREAS SOCIETY, vol. 35, no. 3, 17 July 2020 (2020-07-17), JP , pages A276, XP009537894, ISSN: 0913-0071
RAY PIERRE F, HANDYSIDE ALAN H: "Increasing the denaturation temperature during the first cycles of amplification reduces allele dropout from single cells for preimplantation genetic diagnosis", MOLECULAR HUMAN REPRODUCTION, vol. 2, no. 3, 31 March 1996 (1996-03-31), pages 213 - 218, XP055946833, DOI: 10.1093/molehr/2.3.213
ONO YUSUKE, HAYASHI AKIHIRO, MAEDA CHIHO, SUZUKI MAYUMI, WADA REONA, SATO HIROKI, KAWABATA HIDEMASA, OKADA TETSUHIRO, GOTO TAKUMA,: "Time-saving method for directly amplifying and capturing a minimal amount of pancreatic tumor-derived mutations from fine-needle aspirates using digital PCR", SCIENTIFIC REPORTS, vol. 10, no. 1, 1 December 2020 (2020-12-01), XP055946832, DOI: 10.1038/s41598-020-69221-6
RAY PIERRE F, HANDYSIDE ALAN H: "Increasing the denaturation temperature during the first cycles of amplification reduces allele dropout from single cells for preimplantation genetic diagnosis", MOLECULAR HUMAN REPRODUCTION, vol. 2, no. 3, 31 March 1996 (1996-03-31), pages 213 - 218, XP055946833, DOI: 10.1093/molehr/2.3.213
ONO YUSUKE, HAYASHI AKIHIRO, MAEDA CHIHO, SUZUKI MAYUMI, WADA REONA, SATO HIROKI, KAWABATA HIDEMASA, OKADA TETSUHIRO, GOTO TAKUMA,: "Time-saving method for directly amplifying and capturing a minimal amount of pancreatic tumor-derived mutations from fine-needle aspirates using digital PCR", SCIENTIFIC REPORTS, vol. 10, no. 1, 1 December 2020 (2020-12-01), XP055946832, DOI: 10.1038/s41598-020-69221-6
Attorney, Agent or Firm:
HIROSE Takayuki (JP)
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