Title:
METHOD FOR PRODUCING SHEET-SHAPED CELL CULTURE
Document Type and Number:
WIPO Patent Application WO/2018/168983
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a sheet-shaped cell culture having a high mechanical strength and a method for producing the same. This problem is solved by providing a method for producing a sheet-shaped cell culture, said method comprising (i) a step for, in a cell population containing myoblasts and fibroblasts, controlling the ratio of the both cell species, (ii) a step for seeding the cell population obtained in step (i) on a culture base material, (iii) a step for sheeting, in a cell culture liquid, the cell population seeded in step (ii) and thus forming a sheet-shaped cell culture, and (iv) a step for stripping the sheet-shaped cell culture formed in step (iii) from the culture base material, wherein, in step (ii), the cell population is seeded at such a seeding density that, immediately after the cells, which have been seeded on the culture base material, settle down on the culture base material, 90% or more, relative to the total cells, of the cells are in contact with each other on the culture base material.
Inventors:
NOGUCHI ERI (JP)
Application Number:
PCT/JP2018/010122
Publication Date:
September 20, 2018
Filing Date:
March 15, 2018
Export Citation:
Assignee:
TERUMO CORP (JP)
International Classes:
C12N5/077; A61K35/33; A61K35/34; A61L27/36
Domestic Patent References:
| WO2012118099A1 | 2012-09-07 |
Other References:
HINDS S ET AL.: "Improved tissue culture conditions for engineered skeletal muscle sheets", SCI. WORLD J., vol. 2013, 2013, pages 1 - 6, XP055362178, DOI: doi:10.1155/2013/370151
Attorney, Agent or Firm:
KUZUWA, Kiyoshi (JP)
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