Title:
METHOD FOR PRODUCING SPECIFIC DIFFERENTIATED CELLS
Document Type and Number:
WIPO Patent Application WO/2021/015228
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a method for producing differentiated cells whereby target specific differentiated cells can be efficiently obtained. According to the present invention, provided is a method for producing specific differentiated cells, said method comprising: step (a) for culturing pluripotent stem cells in a medium, which contains any component selected from the group consisting of a histone deacetylase inhibitor, a MAPK/ERK kinase inhibitor, a STAT3 activator, a protein kinase C inhibitor, a Wnt signal inhibitor and a GSK3β inhibitor, to thereby give pluripotent stem cells in which the ratio of 5-methylcytosine to the total cytosines in DNA is lowered compared to the pluripotent stem cells before culturing; and step (b) for culturing the pluripotent stem cells obtained in step (a) in a cytokine-containing medium to give the specific differentiated cells.
Inventors:
YAMAZAKI NAO (JP)
MURAGUCHI TAICHI (JP)
WATANABE SHINICHI (JP)
MURAGUCHI TAICHI (JP)
WATANABE SHINICHI (JP)
Application Number:
PCT/JP2020/028402
Publication Date:
January 28, 2021
Filing Date:
July 22, 2020
Export Citation:
Assignee:
FUJIFILM CORP (JP)
International Classes:
C12N5/071; C12N5/0735; C12N5/10
Domestic Patent References:
| WO2019151386A1 | 2019-08-08 |
Foreign References:
| JP2016521971A | 2016-07-28 | |||
| US20180112187A1 | 2018-04-26 | |||
| JP2011517560A | 2011-06-16 | |||
| JP2017525351A | 2017-09-07 |
Other References:
GUO, G. ET AL.: "Epigenetic resetting of human pluripotency", DEVELOPMENT, vol. 144, 2017, pages 2748 - 2763, XP055456607, DOI: 10.1242/dev.146811
Attorney, Agent or Firm:
SIKS & CO. (JP)
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