Title:
METHOD FOR PRODUCING STANDARD SAMPLE
Document Type and Number:
WIPO Patent Application WO/2015/151511
Kind Code:
A1
Abstract:
Provided is a method for producing a standard sample having a target sequence the same as a DNA fragment having a specific number of copies of the target sequence. After the DNA fragment having the specific number of copies of the target sequence is introduced into cells, the cells are cultured, and the cultured cells are isolated. Thus, a standard sample having a target sequence identical to the DNA fragment having a specific number of copies of the target sequence is produced.
Inventors:
KITTA KAZUMI (JP)
TAKABATAKE REONA (JP)
MANO JUNICHI (JP)
LANG GANG-HUA (JP)
NAKAE HIROKI (JP)
FUTO SATOSHI (JP)
YOSHII JUNJI (JP)
TAKABATAKE REONA (JP)
MANO JUNICHI (JP)
LANG GANG-HUA (JP)
NAKAE HIROKI (JP)
FUTO SATOSHI (JP)
YOSHII JUNJI (JP)
Application Number:
PCT/JP2015/001853
Publication Date:
October 08, 2015
Filing Date:
March 31, 2015
Export Citation:
Assignee:
NAT RES AND DEV AGENCY NAT AGRICULTURE AND FOOD RES ORGANIZATION (JP)
JAPAN MULTIPLEX BIO ANALYSIS CONSORTIUM (JP)
JAPAN MULTIPLEX BIO ANALYSIS CONSORTIUM (JP)
International Classes:
C12N15/09; C12N1/19; C12Q1/02
Domestic Patent References:
| WO2014024493A1 | 2014-02-13 |
Foreign References:
| JP2012125201A | 2012-07-05 |
Other References:
KOEN YOSHISHU: "DNA 1 Bunshi Hyojun Busshitsu no Kaihatsu", JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY, pages 1018
"Evaluating Precision and Accuracy When Quantifying Different Endogenous Control Reference Genes in Maize Using Real-Time PCR", J AGRIC FOOD CHEM, vol. 57, 2009, pages 2903 - 2911, XP055229315
"High Sensitivity Detection and Quantitation of DNA Copy Number and Single Nucleotide Variants with Single Color Droplet Digital PCR", ANAL CHEM, vol. 86, January 2014 (2014-01-01), pages 2618 - 2624, XP055109915
"Evaluating Precision and Accuracy When Quantifying Different Endogenous Control Reference Genes in Maize Using Real-Time PCR", J AGRIC FOOD CHEM, vol. 57, 2009, pages 2903 - 2911, XP055229315
"High Sensitivity Detection and Quantitation of DNA Copy Number and Single Nucleotide Variants with Single Color Droplet Digital PCR", ANAL CHEM, vol. 86, January 2014 (2014-01-01), pages 2618 - 2624, XP055109915
Attorney, Agent or Firm:
OHNO, Seiji et al. (JP)
Seiji Ono (JP)
Seiji Ono (JP)
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