Title:
METHOD FOR PRODUCING TARGET PRODUCT FROM GLYCOLIC ACID UNDER ACTION OF ENZYME
Document Type and Number:
WIPO Patent Application WO/2022/116948
Kind Code:
A1
Abstract:
Disclosed is a method for producing at least one target product from glycolic acid under the action of an enzyme. In order to solve the key problem of losing 25% fixed organic carbon during the recycling process of natural glycolic acid in C3 plants, the present application designs a glycolic acid metabolic pathway comprising an acetate kinase, a phosphoacetyl transferase, a glycolyl coenzyme A reductase and an acetyl phosphate synthase; or the pathway comprises a glycolyl coenzyme A synthase, a glycolyl coenzyme A reductase, an acetyl phosphate synthase and a phosphoacetyl transferase. A new glycolic acid metabolic pathway reduces the loss of organic carbon caused by the recycling process of glycolic acid, and converts 100% of the byproduct glycolic acid generated by photosynthesis into acetyl coenzyme A, thereby providing a new idea for improving the photosynthesis of plants. The pathway also provides a method for preparing glycolaldehyde or acetyl coenzyme A by using glycolic acid as a raw material.
Inventors:
JIANG HUIFENG (CN)
LU XIAOYUN (CN)
LU LINA (CN)
LU XIAOYUN (CN)
LU LINA (CN)
Application Number:
PCT/CN2021/134148
Publication Date:
June 09, 2022
Filing Date:
November 29, 2021
Export Citation:
Assignee:
TIANJIN INST IND BIOTECHNOLOGY CAS (CN)
International Classes:
A01H5/00; C12P9/00; A01H6/38; A01H6/46; A01H6/54; A01H6/82; C12N9/00; C12N9/04; C12N9/10; C12N9/12; C12N15/82; C12P7/24; C12P17/16
Foreign References:
| US20110312049A1 | 2011-12-22 | |||
| CN110564760A | 2019-12-13 |
Other References:
XIAOYUN LU, YUWAN LIU, YIQUN YANG, SHANSHAN WANG, QIAN WANG, XIYA WANG, ZHIHUI YAN, JIAN CHENG, CUI LIU, XUE YANG, HAO LUO, SHENG : "Constructing a synthetic pathway for acetyl-coenzyme A from one-carbon through enzyme design", NATURE COMMUNICATIONS, vol. 10, no. 1, 1 December 2019 (2019-12-01), pages 1 - 10, XP055690259, DOI: 10.1038/s41467-019-09095-z
DEVIN L. TRUDEAU, CHRISTIAN EDLICH-MUTH, JAN ZARZYCKI, MARIEKE SCHEFFEN, MOSHE GOLDSMITH, OLGA KHERSONSKY, ZIV AVIZEMER, SAREL J. : "Design and in vitro realization of carbon-conserving photorespiration", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 115, no. 49, 4 December 2018 (2018-12-04), pages E11455 - E11464, XP055593766, ISSN: 0027-8424, DOI: 10.1073/pnas.1812605115
SCHÜTZE ANDREA, BENNDORF DIRK, PÜTTKER SEBASTIAN, KOHRS FABIAN, BETTENBROCK KATJA: "The Impact of ackA, pta, and ackA-pta Mutations on Growth, Gene Expression and Protein Acetylation in Escherichia coli K-12", FRONTIERS IN MICROBIOLOGY, vol. 11, 1 February 2020 (2020-02-01), pages 1 - 13, XP055870761, DOI: 10.3389/fmicb.2020.00233
DEVIN L. TRUDEAU, CHRISTIAN EDLICH-MUTH, JAN ZARZYCKI, MARIEKE SCHEFFEN, MOSHE GOLDSMITH, OLGA KHERSONSKY, ZIV AVIZEMER, SAREL J. : "Design and in vitro realization of carbon-conserving photorespiration", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 115, no. 49, 4 December 2018 (2018-12-04), pages E11455 - E11464, XP055593766, ISSN: 0027-8424, DOI: 10.1073/pnas.1812605115
SCHÜTZE ANDREA, BENNDORF DIRK, PÜTTKER SEBASTIAN, KOHRS FABIAN, BETTENBROCK KATJA: "The Impact of ackA, pta, and ackA-pta Mutations on Growth, Gene Expression and Protein Acetylation in Escherichia coli K-12", FRONTIERS IN MICROBIOLOGY, vol. 11, 1 February 2020 (2020-02-01), pages 1 - 13, XP055870761, DOI: 10.3389/fmicb.2020.00233
Attorney, Agent or Firm:
BEIJING ORIGINTELLIGENCE IP LAW FIRM (CN)
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