Title:
METHOD FOR PRODUCING THREE-DIMENSIONAL CELL TISSUE
Document Type and Number:
WIPO Patent Application WO/2023/136278
Kind Code:
A1
Abstract:
This method for producing a three-dimensional cell tissue comprises: a step for mixing stromal cells, a cationic substance, an extracellular matrix component and a polymer electrolyte to obtain a mixture; a step for removing the liquid part from the mixture to obtain a cell aggregate; a step for culturing the cell aggregate in a medium to obtain a three-dimensional cell tissue; and a step for culturing the three-dimensional cell tissue in a medium containing ascorbic acid and transforming growth factor-β (TGF-β). The thickness of the three-dimensional cell tissue exceeds 50 μm for at least 3 days after culturing the three-dimensional cell tissue in the medium containing ascorbic acid and TGF-β.
Inventors:
NADA ISANA (JP)
MORIMURA RII (JP)
KITANO SHIRO (JP)
MORIMURA RII (JP)
KITANO SHIRO (JP)
Application Number:
PCT/JP2023/000554
Publication Date:
July 20, 2023
Filing Date:
January 12, 2023
Export Citation:
Assignee:
TOPPAN INC (JP)
International Classes:
C12N5/071
Foreign References:
| JP2020202814A | 2020-12-24 | |||
| JP2011155945A | 2011-08-18 | |||
| JP2019187443A | 2019-10-31 | |||
| JP2019033732A | 2019-03-07 |
Other References:
PATEL BIJAL, XU ZHENGFAN, PINNOCK CAMERON B., KABBANI LOAY S., LAM MAI T.: "Self-assembled Collagen-Fibrin Hydrogel Reinforces Tissue Engineered Adventitia Vessels Seeded with Human Fibroblasts", SCIENTIFIC REPORTS, vol. 8, no. 1, XP093081307, DOI: 10.1038/s41598-018-21681-7
GEESIN J. C., ET AL.: "REGULATION OF COLLAGEN SYNTHESIS IN HUMAN DERMAL FIBROBLASTS IN CONTRACTED COLLAGEN GELS BY ASCORBIC ACID, GROWTH FACTORS, AND INHIBITORS OF LIPID PEROXIDATION.", EXPERIMENTAL CELL RESEARCH, ELSEVIER, AMSTERDAM, NL, vol. 206., no. 02., 1 January 1993 (1993-01-01), AMSTERDAM, NL , pages 283 - 290., XP000909443, ISSN: 0014-4827, DOI: 10.1006/excr.1993.1148
GEESIN J. C., ET AL.: "REGULATION OF COLLAGEN SYNTHESIS IN HUMAN DERMAL FIBROBLASTS IN CONTRACTED COLLAGEN GELS BY ASCORBIC ACID, GROWTH FACTORS, AND INHIBITORS OF LIPID PEROXIDATION.", EXPERIMENTAL CELL RESEARCH, ELSEVIER, AMSTERDAM, NL, vol. 206., no. 02., 1 January 1993 (1993-01-01), AMSTERDAM, NL , pages 283 - 290., XP000909443, ISSN: 0014-4827, DOI: 10.1006/excr.1993.1148
Attorney, Agent or Firm:
MATSUNUMA Yasushi et al. (JP)
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