METHOD FOR PRODUCING TRANSFORMED CELL

WIPO Patent Application WO/2016/163466

A1

The purpose of the present invention is to provide an easy and high-throughput method whereby a DNA can be transferred between different hosts. To achieve this purpose, provided is a method for producing a transformed cell of a yeast or Escherichia coli, said method being characterized in that the yeast or Escherichia coli is transformed using a vector-containing liquid which contains (A) a lytic solution containing a Bacillus subtilis lysate and a vector released therefrom and (B) additive(s) selected from the group consisting of a nuclease inhibitor, a protein-denaturing agent, a nuclease and a combination of two or more of the same.

KANEKO SHINYA (JP)
ITAYA MITSUHIRO (JP)

PCT/JP2016/061420

October 13, 2016

April 07, 2016

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TOKYO INST TECH (JP)
UNIV KEIO (JP)

C12N15/09; C12N1/19; C12N1/21; C40B40/02

JP2000511783A	2000-09-12
JP2002509697A	2002-04-02

"Mitsuhiro Itaya and Shinya Kaneko", NUCLEIC ACIDS RESEARCH, vol. 38, no. 8, 22 March 2010 (2010-03-22), pages 2551 - 2557
TAKASHI AKAMATSU ET AL., JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 114, no. 2, 6 May 2012 (2012-05-06), pages 138 - 143
TOSHIO HARA ET AL., JOURNAL OF THE FACULTY OF AGRICULTURE, vol. 36, no. 1/2, October 1991 (1991-10-01), pages 23 - 28
JERALD S. FEITELSON ET AL., JOURNAL OF GENERAL MICROBIOLOGY, vol. 116, 1 February 1980 (1980-02-01), pages 545 - 547

MORITA Kenichi et al. (JP)
Ken-ichi Morita (JP)

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