Title:
METHOD OF PRODUCING URIDINE 5’-DIPHOSPHO-N-ACETYLGALACTOSAMINE
Document Type and Number:
WIPO Patent Application WO/2006/043525
Kind Code:
A1
Abstract:
A method of producing uridine 5’-diphospho-N-acetylgalactosamine (UDP-GalNAc), which is an important substrate in synthesizing an oligosaccharide, characterized in that in enzymatically producing uridine 5’-diphospho-N-acetylgalactosamine from uridine 5’-triphosphate (UTP) and N-acetylgalactosamine-1-phosphate (GalNAc1-P), uridine 5’-diphospho-N-acetylgalactosamine pyrophosphorylase (UDP-GlcNAc pyrophosphorylase) originating in a microorganism (excluding pathogenic ones) is used as an enzyme. In this method, it is also possible to use, as GalNAc-1P, a product prepared from N-acetylgalactosamine and a phosphate donor in a reaction system with the use of N-acetylgalactosamine kinase. According to this method, uridine 5’-diphospho-N-acetylgalactosamine can be efficiently produced by using a relatively inexpensive substrate.
Inventors:
OKUYAMA KIYOSHI (JP)
NOGUCHI TOSHITADA (JP)
NOGUCHI TOSHITADA (JP)
Application Number:
PCT/JP2005/019081
Publication Date:
April 27, 2006
Filing Date:
October 18, 2005
Export Citation:
Assignee:
YAMASA COPORATION (JP)
OKUYAMA KIYOSHI (JP)
NOGUCHI TOSHITADA (JP)
OKUYAMA KIYOSHI (JP)
NOGUCHI TOSHITADA (JP)
International Classes:
C12P19/30; C12N9/12; C12N15/54; C12N15/62
Domestic Patent References:
| WO1999011810A1 | 1999-03-11 |
Other References:
SUNTHANKAR P ET AL: "Synthesis of 5-azido-UDP-N-acetylhexosamine photoaffinity analogs and radiolabeled UDP-N-acetylhexosamines.", ANAL BIOCHEM., vol. 258, 1998, pages 195 - 201, XP002994974
PASTUSZAK I ET AL: "Identification of the Ga1NAc kinase amino acid sequence.", J BIOL CHEM., vol. 271, no. 39, 1996, pages 23653 - 23656, XP002188778
CHEN X ET AL: "Changing the donor cofactor of bovine alpha 1, 3-galactosyltransferase by fusion with UDP-galactose 4-epimerase.", J BIOL CHEM., vol. 275, no. 41, 2000, pages 31594 - 31600, XP002994975
See also references of EP 1816206A4
PASTUSZAK I ET AL: "Identification of the Ga1NAc kinase amino acid sequence.", J BIOL CHEM., vol. 271, no. 39, 1996, pages 23653 - 23656, XP002188778
CHEN X ET AL: "Changing the donor cofactor of bovine alpha 1, 3-galactosyltransferase by fusion with UDP-galactose 4-epimerase.", J BIOL CHEM., vol. 275, no. 41, 2000, pages 31594 - 31600, XP002994975
See also references of EP 1816206A4
Attorney, Agent or Firm:
Sato, Masaru (3-1 Ariak, Kouto-ku Tokyo 71, JP)
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