Title:
METHOD FOR PRODUCING URSODEOXYCHOLIC ACID USING DEEP EUTECTIC SOLVENT
Document Type and Number:
WIPO Patent Application WO/2021/085997
Kind Code:
A1
Abstract:
The present invention pertains to: a composition for producing ursodeoxycholic acid (UDCA), the composition containing a deep eutectic solvent, a 7 alpha-hydroxy steroid dehydrogenase (7αHSDH), and a 7 beta-hydroxy steroid dehydrogenase (7βHSDH); a UDCA production kit including the composition; and a method for producing UDCA from chenodeoxycholic acid (CDCA) by using 7αHSDH and 7βHSDH in the presence of a reaction solvent including the deep eutectic solvent. A composition for producing UDCA according to the present invention can be used to efficiently produce UDCA from CDCA by using an enzymatic reaction in which the reverse reaction does not occur, and thus can be widely used in the production of various products using UDCA.
Inventors:
KIM HEE TAEK (KR)
RYU MI HEE (KR)
JUNG YE JEAN (KR)
SONG BONG KEUN (KR)
KANG KYOUNG HEE (KR)
RYU MI HEE (KR)
JUNG YE JEAN (KR)
SONG BONG KEUN (KR)
KANG KYOUNG HEE (KR)
Application Number:
PCT/KR2020/014774
Publication Date:
May 06, 2021
Filing Date:
October 28, 2020
Export Citation:
Assignee:
KOREA RES INST CHEMICAL TECH (KR)
International Classes:
C12P33/00; C12N9/04
Foreign References:
| US20090117628A1 | 2009-05-07 | |||
| CN105861613A | 2016-08-17 |
Other References:
CHEN XI, CUI YUNFENG, FENG JINHUI, WANG YU, LIU XIANGTAO, WU QIAQING, ZHU DUNMING, MA YANHE: "Flavin Oxidoreductase‐Mediated Regeneration of Nicotinamide Adenine Dinucleotide with Dioxygen and Catalytic Amount of Flavin Mononucleotide for One‐Pot Multi‐Enzymatic Preparation of Ursodeoxycholic Acid", ADVANCED SYNTHESIS AND CATALYSIS, XP055807194, ISSN: 1615-4150, DOI: 10.1002/adsc.201900111
MING-MIN ZHENG, FEI-FEI CHEN, HAO LI, CHUN-XIU LI, JIAN-HE XU: "Continuous Production of Ursodeoxycholic Acid by Using Two Cascade Reactors with Co-immobilized Enzymes", CHEMBIOCHEM, vol. 19, no. 4, 16 February 2018 (2018-02-16), pages 347 - 353, XP055711552, ISSN: 1439-4227, DOI: 10.1002/cbic.201700415
FABIO TONIN, ISABEL W C E ARENDS: "Latest development in the synthesis of ursodeoxycholic acid (UDCA): a critical review", BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY, vol. 14, pages 470 - 483, XP055657131, DOI: 10.3762/bjoc.14.33
MING-MIN ZHENG, FEI-FEI CHEN, HAO LI, CHUN-XIU LI, JIAN-HE XU: "Continuous Production of Ursodeoxycholic Acid by Using Two Cascade Reactors with Co-immobilized Enzymes", CHEMBIOCHEM, vol. 19, no. 4, 16 February 2018 (2018-02-16), pages 347 - 353, XP055711552, ISSN: 1439-4227, DOI: 10.1002/cbic.201700415
FABIO TONIN, ISABEL W C E ARENDS: "Latest development in the synthesis of ursodeoxycholic acid (UDCA): a critical review", BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY, vol. 14, pages 470 - 483, XP055657131, DOI: 10.3762/bjoc.14.33
Attorney, Agent or Firm:
HANOL INTELLECTUAL PROPERTY AND LAW (KR)
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