Title:
METHOD FOR PRODUCING VIRUS-SPECIFIC CTL
Document Type and Number:
WIPO Patent Application WO/2008/023786
Kind Code:
A1
Abstract:
It is intended to provide a closed culture system of virus-specific CTL which has
convenience and safety features and can be implemented at low cost for the purpose
of achieving the practical application of cell therapy with the use of CTL specific
to a virus (particularly HCMV). After virus-specific CTL epitope peptide and
peripheral blood mononuclear cells are mixed and incubated, restimulation with
this epitope peptide is given in a medium. Then, virus-specific CTL is isolated by
targeting freshly expressed CD137 antigen, and T cells proliferated from peripheral
blood mononuclear cells by the stimulation with OKT3 (anti-CD3 monoclonal antibody)
are brought into contact with the epitope peptide in the medium. Then, the T cells
and the virus-specific CTL are cocultured, whereby the virus-specific CTL is
proliferated.
Inventors:
SUZUKI, Susumu (C/O MEDICAL & BIOLOGICAL LABORATORIES CO. LTD, Sumitomoshoji-Marunouchi Bldg. 5F, 5-10, Marunouchi 3-chome, Naka-ku, Nagoya-sh, Aichi 02, 4620002, JP)
鈴木 進 (〒02 愛知県名古屋市中区丸の内３丁目５番１０号 住友商事丸の内ビル５Ｆ 株式会社医学生物学研究所内 Aichi, 4620002, JP)
WATANABE, Kazue (C/O MEDICAL & BIOLOGICAL LABORATORIES CO. LTD, Sumitomoshoji-Marunouchi Bldg. 5F, 5-10, Marunouchi 3-chome, Naka-ku, Nagoya-sh, Aichi 02, 4600002, JP)
渡邉 一絵 (〒02 愛知県名古屋市中区丸の内三丁目５番１０号 住友商事丸の内ビル５階 株式会社医学生物学研究所内 Aichi, 4600002, JP)
TAJI, Shingo (C/O MEDICAL & BIOLOGICAL LABORATORIES CO. LTD, Sumitomoshoji-Marunouchi Bldg. 5F, 5-10, Marunouchi 3-chome, Naka-ku, Nagoya-sh, Aichi 02, 4600002, JP)
田路 真悟 (〒02 愛知県名古屋市中区丸の内３丁目５番１０号 住友商事丸の内ビル５Ｆ 株式会社医学生物学研究所内 Aichi, 4600002, JP)
KIN, Ritsuko (C/O MEDICAL & BIOLOGICAL LABORATORIES CO. LTD, Sumitomoshoji-Marunouchi Bldg. 5F, 5-10, Marunouchi 3-chome, Naka-ku, Nagoya-sh, Aichi 02, 4600002, JP)
鈴木 進 (〒02 愛知県名古屋市中区丸の内３丁目５番１０号 住友商事丸の内ビル５Ｆ 株式会社医学生物学研究所内 Aichi, 4620002, JP)
WATANABE, Kazue (C/O MEDICAL & BIOLOGICAL LABORATORIES CO. LTD, Sumitomoshoji-Marunouchi Bldg. 5F, 5-10, Marunouchi 3-chome, Naka-ku, Nagoya-sh, Aichi 02, 4600002, JP)
渡邉 一絵 (〒02 愛知県名古屋市中区丸の内三丁目５番１０号 住友商事丸の内ビル５階 株式会社医学生物学研究所内 Aichi, 4600002, JP)
TAJI, Shingo (C/O MEDICAL & BIOLOGICAL LABORATORIES CO. LTD, Sumitomoshoji-Marunouchi Bldg. 5F, 5-10, Marunouchi 3-chome, Naka-ku, Nagoya-sh, Aichi 02, 4600002, JP)
田路 真悟 (〒02 愛知県名古屋市中区丸の内３丁目５番１０号 住友商事丸の内ビル５Ｆ 株式会社医学生物学研究所内 Aichi, 4600002, JP)
KIN, Ritsuko (C/O MEDICAL & BIOLOGICAL LABORATORIES CO. LTD, Sumitomoshoji-Marunouchi Bldg. 5F, 5-10, Marunouchi 3-chome, Naka-ku, Nagoya-sh, Aichi 02, 4600002, JP)
Application Number:
JP2007/066439
Publication Date:
February 28, 2008
Filing Date:
August 24, 2007
Export Citation:
Assignee:
MEDICAL & BIOLOGICAL LABORATORIES CO., LTD. (Sumitomoshoji-Marunouchi Bldg. 5F, 5-10 Marunouchi 3-chome, Naka-ku, Nagoya-sh, Aichi 02, 4600002, JP)
株式会社医学生物学研究所 (〒02 愛知県名古屋市中区丸の内三丁目５番１０号 住友商事丸の内ビル５階 Aichi, 4600002, JP)
AICHI-ken (1-2 Sannomaru 3-chome, Naka-ku Nagoya-sh, Aichi 01, 4600001, JP)
愛知県 (〒01 愛知県名古屋市中区三の丸三丁目１番２号 Aichi, 4600001, JP)
SUZUKI, Susumu (C/O MEDICAL & BIOLOGICAL LABORATORIES CO. LTD, Sumitomoshoji-Marunouchi Bldg. 5F, 5-10, Marunouchi 3-chome, Naka-ku, Nagoya-sh, Aichi 02, 4620002, JP)
鈴木 進 (〒02 愛知県名古屋市中区丸の内３丁目５番１０号 住友商事丸の内ビル５Ｆ 株式会社医学生物学研究所内 Aichi, 4620002, JP)
WATANABE, Kazue (C/O MEDICAL & BIOLOGICAL LABORATORIES CO. LTD, Sumitomoshoji-Marunouchi Bldg. 5F, 5-10, Marunouchi 3-chome, Naka-ku, Nagoya-sh, Aichi 02, 4600002, JP)
渡邉 一絵 (〒02 愛知県名古屋市中区丸の内三丁目５番１０号 住友商事丸の内ビル５階 株式会社医学生物学研究所内 Aichi, 4600002, JP)
TAJI, Shingo (C/O MEDICAL & BIOLOGICAL LABORATORIES CO. LTD, Sumitomoshoji-Marunouchi Bldg. 5F, 5-10, Marunouchi 3-chome, Naka-ku, Nagoya-sh, Aichi 02, 4600002, JP)
株式会社医学生物学研究所 (〒02 愛知県名古屋市中区丸の内三丁目５番１０号 住友商事丸の内ビル５階 Aichi, 4600002, JP)
AICHI-ken (1-2 Sannomaru 3-chome, Naka-ku Nagoya-sh, Aichi 01, 4600001, JP)
愛知県 (〒01 愛知県名古屋市中区三の丸三丁目１番２号 Aichi, 4600001, JP)
SUZUKI, Susumu (C/O MEDICAL & BIOLOGICAL LABORATORIES CO. LTD, Sumitomoshoji-Marunouchi Bldg. 5F, 5-10, Marunouchi 3-chome, Naka-ku, Nagoya-sh, Aichi 02, 4620002, JP)
鈴木 進 (〒02 愛知県名古屋市中区丸の内３丁目５番１０号 住友商事丸の内ビル５Ｆ 株式会社医学生物学研究所内 Aichi, 4620002, JP)
WATANABE, Kazue (C/O MEDICAL & BIOLOGICAL LABORATORIES CO. LTD, Sumitomoshoji-Marunouchi Bldg. 5F, 5-10, Marunouchi 3-chome, Naka-ku, Nagoya-sh, Aichi 02, 4600002, JP)
渡邉 一絵 (〒02 愛知県名古屋市中区丸の内三丁目５番１０号 住友商事丸の内ビル５階 株式会社医学生物学研究所内 Aichi, 4600002, JP)
TAJI, Shingo (C/O MEDICAL & BIOLOGICAL LABORATORIES CO. LTD, Sumitomoshoji-Marunouchi Bldg. 5F, 5-10, Marunouchi 3-chome, Naka-ku, Nagoya-sh, Aichi 02, 4600002, JP)
International Classes:
A61K35/14; A61P31/12; C12N5/0783; C12Q1/02
Attorney, Agent or Firm:
KOBAYASHI, Youhei (KY International Patent Firm, Win's Bldg. 2F.4-44, Chuou-ch, Kuwana-shi Mie 68, 5110068, JP)
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