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Title:
METHOD FOR PRODUCING WHOLE PLANTS FROM PROTOPLASTS
Document Type and Number:
WIPO Patent Application WO/2017/061806
Kind Code:
A1
Abstract:
The present invention relates to method for preparing a plant from a protoplast comprising knocking-out or knocking-in one or more the endogenous gene of the protoplast, and the plant regenerated from a genome-modified protoplast prepared by the above method.

Inventors:
KIM JIN SOO (KR)
KIM JUNGEUN (KR)
CHOE SUNGHWA (KR)
WOO JE WOOK (KR)
KWON SOON IL (KR)
Application Number:
PCT/KR2016/011217
Publication Date:
April 13, 2017
Filing Date:
October 06, 2016
Export Citation:
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Assignee:
INST BASIC SCIENCE (KR)
UNIV SEOUL NAT R & DB FOUND (KR)
AICT (KR)
International Classes:
C12N15/82; A01H5/12; C12N9/22
Domestic Patent References:
WO2014199358A12014-12-18
Foreign References:
CN102618554A2012-08-01
US20140295556A12014-10-02
US20090013433A12009-01-08
KR20160011216A2016-01-29
Other References:
HYUN ET AL.: "Site-directed mutagenesis in Arabidopsis thaliana using dividing tissue-targeted RGEN of the CRISPR/Cas system to generate heritable null alleles", PLANTA, vol. 241, pages 271 - 284, XP035417502
YANG ET AL.: "The mechanisms of brassinosteroids' action: from signal transduction to plant development", MOLECULAR PLANT, vol. 4, no. 4, July 2011 (2011-07-01), pages 588 - 600, XP055373079
See also references of EP 3359674A4
KIM, H., NAT REV GENET, vol. 15, 2014, pages 321 - 334
JONES, H.D., NATURE PLANTS, vol. 1, 2015, pages 14011
KIM, S, GENOME RESEARCH, vol. 24, 2014, pages 1012 - 1019
VOYTAS, D.F.: "Plant genome engineering with sequence-specific nucleases", ANNUAL REVIEW OF PLANT BIOLOGY, vol. 64, 2013, pages 327 - 350, XP002729239, DOI: 10.1146/annurev-arplant-042811-105552
BELHAJ, K.CHAPARRO-GARCIA, A.KAMOUN, S.NEKRASOV, V.: "Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system", PLANT METHODS, vol. 9, no. 1, 2013, pages 1 - 10, XP021164954, DOI: 10.1186/1746-4811-9-39
Attorney, Agent or Firm:
SON, Min (KR)
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