Title:
METHOD FOR PRODUCING WHOLE PLANTS FROM PROTOPLASTS
Document Type and Number:
WIPO Patent Application WO/2017/061806
Kind Code:
A1
Abstract:
The present invention relates to method for preparing a plant from a protoplast comprising knocking-out or knocking-in one or more the endogenous gene of the protoplast, and the plant regenerated from a genome-modified protoplast prepared by the above method.
Inventors:
KIM JIN SOO (KR)
KIM JUNGEUN (KR)
CHOE SUNGHWA (KR)
WOO JE WOOK (KR)
KWON SOON IL (KR)
KIM JUNGEUN (KR)
CHOE SUNGHWA (KR)
WOO JE WOOK (KR)
KWON SOON IL (KR)
Application Number:
PCT/KR2016/011217
Publication Date:
April 13, 2017
Filing Date:
October 06, 2016
Export Citation:
Assignee:
INST BASIC SCIENCE (KR)
UNIV SEOUL NAT R & DB FOUND (KR)
AICT (KR)
UNIV SEOUL NAT R & DB FOUND (KR)
AICT (KR)
International Classes:
C12N15/82; A01H5/12; C12N9/22
Domestic Patent References:
WO2014199358A1 | 2014-12-18 |
Foreign References:
CN102618554A | 2012-08-01 | |||
US20140295556A1 | 2014-10-02 | |||
US20090013433A1 | 2009-01-08 | |||
KR20160011216A | 2016-01-29 |
Other References:
HYUN ET AL.: "Site-directed mutagenesis in Arabidopsis thaliana using dividing tissue-targeted RGEN of the CRISPR/Cas system to generate heritable null alleles", PLANTA, vol. 241, pages 271 - 284, XP035417502
YANG ET AL.: "The mechanisms of brassinosteroids' action: from signal transduction to plant development", MOLECULAR PLANT, vol. 4, no. 4, July 2011 (2011-07-01), pages 588 - 600, XP055373079
See also references of EP 3359674A4
KIM, H., NAT REV GENET, vol. 15, 2014, pages 321 - 334
JONES, H.D., NATURE PLANTS, vol. 1, 2015, pages 14011
KIM, S, GENOME RESEARCH, vol. 24, 2014, pages 1012 - 1019
VOYTAS, D.F.: "Plant genome engineering with sequence-specific nucleases", ANNUAL REVIEW OF PLANT BIOLOGY, vol. 64, 2013, pages 327 - 350, XP002729239, DOI: 10.1146/annurev-arplant-042811-105552
BELHAJ, K.CHAPARRO-GARCIA, A.KAMOUN, S.NEKRASOV, V.: "Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system", PLANT METHODS, vol. 9, no. 1, 2013, pages 1 - 10, XP021164954, DOI: 10.1186/1746-4811-9-39
YANG ET AL.: "The mechanisms of brassinosteroids' action: from signal transduction to plant development", MOLECULAR PLANT, vol. 4, no. 4, July 2011 (2011-07-01), pages 588 - 600, XP055373079
See also references of EP 3359674A4
KIM, H., NAT REV GENET, vol. 15, 2014, pages 321 - 334
JONES, H.D., NATURE PLANTS, vol. 1, 2015, pages 14011
KIM, S, GENOME RESEARCH, vol. 24, 2014, pages 1012 - 1019
VOYTAS, D.F.: "Plant genome engineering with sequence-specific nucleases", ANNUAL REVIEW OF PLANT BIOLOGY, vol. 64, 2013, pages 327 - 350, XP002729239, DOI: 10.1146/annurev-arplant-042811-105552
BELHAJ, K.CHAPARRO-GARCIA, A.KAMOUN, S.NEKRASOV, V.: "Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system", PLANT METHODS, vol. 9, no. 1, 2013, pages 1 - 10, XP021164954, DOI: 10.1186/1746-4811-9-39
Attorney, Agent or Firm:
SON, Min (KR)
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