Title:
METHOD FOR PRODUCTION OF RECOMBINANT HUMAN IDURONATE 2-SULFATASE
Document Type and Number:
WIPO Patent Application WO/2012/101998
Kind Code:
A1
Abstract:
Disclosed is a method for production of recombinant human iduronate 2-sulfatase (rhI2S) in a large scale, with a high purity, and mannose 6-phosphate residues. The method comprises the steps of (a) culturing rhI2S-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to cation-exchange column chromatography, (d) to dye affinity column chromatography, (e) to anion-exchange column chromatography, and (f) to a column chromatography employing as solid phase a material having affinity for phosphate group, and (g) to gel filtration column chromatography, in the order.
Inventors:
MIHARA KAZUTOSHI (JP)
KAWASAKI ATSUKO (JP)
OOTSUKI KOUTA (JP)
HATANO YUUKICHI (JP)
KAMEI SHOICHIRO (JP)
SUGIMURA ATSUSHI (JP)
KAWASAKI ATSUKO (JP)
OOTSUKI KOUTA (JP)
HATANO YUUKICHI (JP)
KAMEI SHOICHIRO (JP)
SUGIMURA ATSUSHI (JP)
Application Number:
PCT/JP2012/000365
Publication Date:
August 02, 2012
Filing Date:
January 23, 2012
Export Citation:
Assignee:
JAPAN CHEM RES (JP)
MIHARA KAZUTOSHI (JP)
KAWASAKI ATSUKO (JP)
OOTSUKI KOUTA (JP)
HATANO YUUKICHI (JP)
KAMEI SHOICHIRO (JP)
SUGIMURA ATSUSHI (JP)
MIHARA KAZUTOSHI (JP)
KAWASAKI ATSUKO (JP)
OOTSUKI KOUTA (JP)
HATANO YUUKICHI (JP)
KAMEI SHOICHIRO (JP)
SUGIMURA ATSUSHI (JP)
International Classes:
C12N15/09; C12N9/16
Domestic Patent References:
| WO1994012650A2 | 1994-06-09 | |||
| WO1995031560A1 | 1995-11-23 |
Foreign References:
| JP2010511378A | 2010-04-15 | |||
| JP2009273427A | 2009-11-26 | |||
| US5728381A | 1998-03-17 | |||
| US5932211A | 1999-08-03 | |||
| US20040229250A1 | 2004-11-18 |
Other References:
WASTESON A. ET AL.: "Iduronate sulfatase from human plasma", METHODS ENZYMOL., vol. 83, 1982, pages 573 - 578, XP009177144
DI NATALE P. ET AL.: "Iduronate sulfatase from human placenta", BIOCHIM.BIOPHYS.ACTA, vol. 839, no. 3, 1985, pages 258 - 61, XP025789183
BIELICKI J. ET AL.: "Recombinant human iduronate-2-sulphatase:correction of mucopolysaccharidosis-type II fibroblasts and characterization of the purified enzyme", BIOCHEM.J., vol. 289, no. 1, 1993, pages 241 - 246, XP055081180
YUTAKA T. ET AL.: "Purification and some properties of human liver iduronate sulfatase", BIOCHEM., vol. 91, no. 2, 1982, pages 433 - 41, XP009177168
BIELICKI J. ET AL.: "Human liver iduronate-2-sulphatase. Purification, characterization and catalytic properties", BIOCHEM.J., vol. 271, no. 1, 1990, pages 75 - 86, XP002575045
See also references of EP 2668272A4
WASTESON A. ET AL.: "Iduronate sulfatase from human plasma", METHODS ENZYMOL., vol. 83, 1982, pages 573 - 578
BIELICKI J. ET AL.: "Recombinant human iduronate-2-sulphatase:correction of mucopolysaccharidosis-type II fibroblasts and characterization of the purified enzyme", BIOCHEM.J., vol. 289, no. 1, 1993, pages 241 - 246, XP055081180, DOI: doi:10.1042/bj2890241
WILSON PJ ET AL., PROC NATL ACAD SCI USA., vol. 87, 1990, pages 8531 - 5
DEAN MF ET AL., J CLIN INVEST., vol. 63, 1979, pages 138 - 45
BROWN FR 3 ET AL., AM J MED GENET., vol. 13, 1982, pages 309 - 18
TSUKIMURA T., BIOL PHARM BULL., vol. 31, 1979, pages 1691 - 5
DI NATALE P. ET AL.: "Iduronate sulfatase from human placenta", BIOCHIM.BIOPHYS.ACTA, vol. 839, no. 3, 1985, pages 258 - 61, XP025789183
BIELICKI J. ET AL.: "Recombinant human iduronate-2-sulphatase:correction of mucopolysaccharidosis-type II fibroblasts and characterization of the purified enzyme", BIOCHEM.J., vol. 289, no. 1, 1993, pages 241 - 246, XP055081180
YUTAKA T. ET AL.: "Purification and some properties of human liver iduronate sulfatase", BIOCHEM., vol. 91, no. 2, 1982, pages 433 - 41, XP009177168
BIELICKI J. ET AL.: "Human liver iduronate-2-sulphatase. Purification, characterization and catalytic properties", BIOCHEM.J., vol. 271, no. 1, 1990, pages 75 - 86, XP002575045
See also references of EP 2668272A4
WASTESON A. ET AL.: "Iduronate sulfatase from human plasma", METHODS ENZYMOL., vol. 83, 1982, pages 573 - 578
BIELICKI J. ET AL.: "Recombinant human iduronate-2-sulphatase:correction of mucopolysaccharidosis-type II fibroblasts and characterization of the purified enzyme", BIOCHEM.J., vol. 289, no. 1, 1993, pages 241 - 246, XP055081180, DOI: doi:10.1042/bj2890241
WILSON PJ ET AL., PROC NATL ACAD SCI USA., vol. 87, 1990, pages 8531 - 5
DEAN MF ET AL., J CLIN INVEST., vol. 63, 1979, pages 138 - 45
BROWN FR 3 ET AL., AM J MED GENET., vol. 13, 1982, pages 309 - 18
TSUKIMURA T., BIOL PHARM BULL., vol. 31, 1979, pages 1691 - 5
Attorney, Agent or Firm:
HAYASAKA, Takumi (7th Fl. Kitahama-heiwa Bldg., 5-13, Kitahama 2-chome, Chuo-ku, Osaka-sh, Osaka 41, JP)
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