Title:
METHOD FOR PROMOTING ENZYMOLYSIS OF LIGNOCELLULOSE BY USING PH-RESPONSIVE LIGNIN AMPHOTERIC SURFACTANT AND RECOVERY OF CELLULASE
Document Type and Number:
WIPO Patent Application WO/2018/214445
Kind Code:
A1
Abstract:
A method for promoting enzymolysis of lignocellulose by using a pH-responsive lignin amphoteric surfactant and recovery of a cellulase. The method comprises: adding lignocellulose to a buffer solution, and then adding a pH-responsive lignin amphoteric surfactant and a cellulase; controlling the pH of the mixed solution to be 4.0-6.2, heating the solution to a temperature of 40-60°C and reacting at said temperature for 24-96 h to obtain a saccharified hydrolyzate of lignocellulose; and obtaining an enzymolysed liquid by solid-liquid separation, and then adjusting the pH of the enzymolysed liquid to precipitate the pH-responsive lignin amphoteric surfactant and the cellulase for recycling. The method can effectively improve the enzymolysis efficiency of lignocellulose, recover a certain amount of cellulase, and expand the applications of industrial lignin. The method does not require additional equipment, and is simple to operate and environmentally friendly.
Inventors:
LOU HONGMING (CN)
CAI CHENG (CN)
QIU XUEQING (CN)
ZHAN XUEJUAN (CN)
YANG DONGJIE (CN)
PANG YUXIA (CN)
CAI CHENG (CN)
QIU XUEQING (CN)
ZHAN XUEJUAN (CN)
YANG DONGJIE (CN)
PANG YUXIA (CN)
Application Number:
PCT/CN2017/113139
Publication Date:
November 29, 2018
Filing Date:
November 27, 2017
Export Citation:
Assignee:
UNIV SOUTH CHINA TECH (CN)
International Classes:
C12P19/14; C12N9/42; C12P7/06
Domestic Patent References:
| WO2016077942A1 | 2016-05-26 |
Foreign References:
| CN101906450A | 2010-12-08 | |||
| CN102517359A | 2012-06-27 | |||
| CN106520845A | 2017-03-22 | |||
| CN101824436A | 2010-09-08 |
Attorney, Agent or Firm:
GUANGZHOU HUAXUE INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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