Title:
METHOD FOR PROMOTING EXPRESSION OF FGF-2 IN 293 T CELLS BY MEANS OF ENHANCER
Document Type and Number:
WIPO Patent Application WO/2020/253400
Kind Code:
A1
Abstract:
Provided is an enhancer sequence. The enhancer sequence comprises the DNA sequence of an NF-κB binding site and the DNA sequence of a CREB binding site. Provided are also a plasmid vector pNC1 containing the enhancer sequence, a method for preparing the plasmid vector pNC1, the use of the plasmid vector pNC1 in expressing FGF-2, and a transformant containing the plasmid vector pNC1. The plasmid vector pNC1 can significantly increase the expression level of FGF-2, and the expressed FGF-2 is substantially soluble, and the bioactivity thereof is comparable to that of a commercially available FGF-2.
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Inventors:
CHUNG SHU KUN (CN)
KWONG WAI YEUNG (CN)
KWONG WAI YEUNG (CN)
Application Number:
PCT/CN2020/087967
Publication Date:
December 24, 2020
Filing Date:
April 30, 2020
Export Citation:
Assignee:
DREAMTEC INTELLECTUAL PROPERTY LTD (CN)
International Classes:
C12N15/85; C12N5/10; C12N15/113; C12N15/12; C12N15/67
Foreign References:
| CN110777165A | 2020-02-11 | |||
| CN110777148A | 2020-02-11 | |||
| CN102787137A | 2012-11-21 |
Other References:
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SHI, TONG ET AL.: "Establishment of a HEK-293 Cell Line Stably Expressing Human TLR5 And NF-κB Responsive Reporter", JOURNAL OF INTERNATIONAL PHARMACEUTICAL RESEARCH, vol. 44, no. 5, 31 May 2017 (2017-05-31), ISSN: 1674-0440, DOI: 20200730141011Y
ANNELEEN SPOOREN ET AL.: "Cooperation of NFκB and CREB to induce synergistic IL-6 expression in astrocytes", CELLULAR SIGNALLING, vol. 22, 25 January 2010 (2010-01-25), XP026907126, ISSN: 1873-3913, DOI: 20200729182928X
YIQUN DU ET AL.: "NF-κB and Enhancer-binding CREB Protein Scaffolded by CREB-binding Protein (CBP)/p300 Proteins Regulate CD59 Protein Expression to Protect Cells from Complement Attack", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 289, no. 5, 31 January 2014 (2014-01-31), XP055766753, ISSN: 0021-9258, DOI: 20200729182256A
MI OK KIM ET AL.: "PKA and cAMP stimulate proliferation of mouse embryonic stem cells by elevating GLUT1 expression mediated by the NF-κB and CREB/CBP signaling pathways", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1820, 29 May 2012 (2012-05-29), XP028449224, ISSN: 0304-4165, DOI: 20200729183218A
"UniProtKB/Swiss-Port: P09038.3", UNIPROTKB/SWISS-PORT DATABASE, 13 February 2019 (2019-02-13), DOI: 20200729185126A
SHI, TONG ET AL.: "Establishment of a HEK-293 Cell Line Stably Expressing Human TLR5 And NF-κB Responsive Reporter", JOURNAL OF INTERNATIONAL PHARMACEUTICAL RESEARCH, vol. 44, no. 5, 31 May 2017 (2017-05-31), ISSN: 1674-0440, DOI: 20200730141011Y
ANNELEEN SPOOREN ET AL.: "Cooperation of NFκB and CREB to induce synergistic IL-6 expression in astrocytes", CELLULAR SIGNALLING, vol. 22, 25 January 2010 (2010-01-25), XP026907126, ISSN: 1873-3913, DOI: 20200729182928X
YIQUN DU ET AL.: "NF-κB and Enhancer-binding CREB Protein Scaffolded by CREB-binding Protein (CBP)/p300 Proteins Regulate CD59 Protein Expression to Protect Cells from Complement Attack", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 289, no. 5, 31 January 2014 (2014-01-31), XP055766753, ISSN: 0021-9258, DOI: 20200729182256A
MI OK KIM ET AL.: "PKA and cAMP stimulate proliferation of mouse embryonic stem cells by elevating GLUT1 expression mediated by the NF-κB and CREB/CBP signaling pathways", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1820, 29 May 2012 (2012-05-29), XP028449224, ISSN: 0304-4165, DOI: 20200729183218A
"UniProtKB/Swiss-Port: P09038.3", UNIPROTKB/SWISS-PORT DATABASE, 13 February 2019 (2019-02-13), DOI: 20200729185126A
Attorney, Agent or Firm:
VALLEY OF SOFT BEIJING INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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