Title:
METHOD FOR PROMOTING SOMATIC CELL REPROGRAMMING, AND CELL PREPARATION KIT
Document Type and Number:
WIPO Patent Application WO/2014/058080
Kind Code:
A1
Abstract:
This method for promoting somatic cell reprogramming includes a culturing step for culturing somatic cells in a state of contact with a non-biological substrate, the elastic modulus of which is in the range of 0.1 kPa to 200 kPa, in the presence of at least one factor selected from the factor group consisting of a histone deacetylase inhibitor, a TGF-β receptor inhibitor, a MAPK/ERK pathway inhibitor, and a 3'-phosphoinositide-dependent protein kinase-1 activator.
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Inventors:
FUJITA HIDEAKI (JP)
WATANABE TOMONOBU (JP)
HIGUCHI SAYAKA (JP)
WATANABE TOMONOBU (JP)
HIGUCHI SAYAKA (JP)
Application Number:
PCT/JP2013/082246
Publication Date:
April 17, 2014
Filing Date:
November 29, 2013
Export Citation:
Assignee:
RIKEN (JP)
International Classes:
C12N5/071; C12N1/00
Domestic Patent References:
| WO2011123572A1 | 2011-10-06 | |||
| WO2011047300A1 | 2011-04-21 | |||
| WO2011072461A1 | 2011-06-23 |
Other References:
HASSANI SEYEDEH-NAFISEH ET AL.: "Simultaneous Suppression of TGF-P and ERK Signaling Contributes to the Highly Efficient and Reproducible Generation of Mouse Embryonic Stem Cells from Previously Considered Refractory and Non-permissive Strains", STEM CELL REV. AND REP., vol. 8, no. 2, 2011, pages 472 - 481
ZHU SAIYONG ET AL.: "Reprogramming of Human Primary Somatic Cells by OCT4 and Chemical Compounds", CELL STEM CELL, vol. 7, no. 6, 2010, pages 651 - 655
KUNIO HIRANO ET AL.: "Human and Mouse Induced Pluripotent Stem Cells Are Differentially Reprogrammed in Response to Kinase Inhibitors", STEM CELLS AND DEVELOPMENT, vol. 21, no. 8, 2011, pages 1287 - 1298
YU JUNYING ET AL.: "Efficient feeder-free episomal reprogramming with small molecules. art. e17557", PLOS ONE, vol. 6, no. 3, 2011, pages 1 - 10
ZHU SAIYONG ET AL.: "Reprogramming of Human Primary Somatic Cells by OCT4 and Chemical Compounds", CELL STEM CELL, vol. 7, no. 6, 2010, pages 651 - 655
KUNIO HIRANO ET AL.: "Human and Mouse Induced Pluripotent Stem Cells Are Differentially Reprogrammed in Response to Kinase Inhibitors", STEM CELLS AND DEVELOPMENT, vol. 21, no. 8, 2011, pages 1287 - 1298
YU JUNYING ET AL.: "Efficient feeder-free episomal reprogramming with small molecules. art. e17557", PLOS ONE, vol. 6, no. 3, 2011, pages 1 - 10
Attorney, Agent or Firm:
HARAKENZO WORLD PATENT & TRADEMARK (JP)
Patent business corporation Hara [Kenzo] international patent firm (JP)
Patent business corporation Hara [Kenzo] international patent firm (JP)
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