Title:
METHOD FOR PURIFICATION AND PREPARATION OF ALLERGEN POSITIVE SERUM
Document Type and Number:
WIPO Patent Application WO/2016/058237
Kind Code:
A1
Abstract:
Disclosed is a method for purification and preparation of an allergen positive serum, comprising the following steps: immunizing a healthy animal using an allergen, collecting blood samples, and obtaining an antiserum; and purifying the antiserum to obtain a positive serum. The particular method for purification is as follows: carrying out affinity purification on the antiserum to obtain an IgG antibody; coupling the IgG antibody and human IgE Fc in a mass ratio of 1 : 1-2, separating and purifying to obtain a concentrated IgG-IgE Fc coupler solution, and diluting the concentrated solution to a concentration of 0.5-1 μg/mL to obtain the positive serum. The method can be used for batch preparing positive serums of various allergens, which can be used for positive quality control in the process of preparing the detection kit, and can also be used as a production raw material for calibrators, and the preparation method is simple and easy to implement.
Inventors:
WANG HONG (CN)
Application Number:
PCT/CN2014/091114
Publication Date:
April 21, 2016
Filing Date:
November 14, 2014
Export Citation:
Assignee:
HOB BIOTECH GROUP SUZHOU CO LTD (CN)
International Classes:
G01N33/96; G01N33/531
Domestic Patent References:
| WO2013039450A1 | 2013-03-21 | |||
| WO2008086721A1 | 2008-07-24 |
Foreign References:
| US4929543A | 1990-05-29 | |||
| CN1924579A | 2007-03-07 | |||
| US5895811A | 1999-04-20 | |||
| CN102809657A | 2012-12-05 | |||
| US5478753A | 1995-12-26 | |||
| CN103926396A | 2014-07-16 |
Attorney, Agent or Firm:
SUZHOU GROUNZE INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
苏州广正知识产权代理有限公司 (CN)
苏州广正知识产权代理有限公司 (CN)
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