Title:
METHOD OF PURIFYING PLASMINOGEN
Document Type and Number:
WIPO Patent Application WO/1995/004077
Kind Code:
A1
Abstract:
Plasminogen is purified by bringing a plasminogen-containing composition into contact with an anion exchanger and recovering the unadsorbed fraction. The contact is performed by adjusting the concentration of a salt such as sodium chloride to 0.1-2 w/v%, preferably to 0.4-0.8 w/v%, and, if desirable, adjusting the pH to 6 to 8. This method is useful to remove contaminants, in particular, a high-molecular substance having a plasminogen anti-genicity, from a plasminogen-containing composition. Further it permits plasminogen to be purified in a short time efficiently in a good yield at a low cost. Therefore this method is advantageous for preparing pure plasminogen from a large quantity of a plasminogen-containing composition and is extremely useful as a process for producing plasminogen on an industrial scale.
Inventors:
MOCHIZUKI SHINOBU (JP)
KOBAYASHI TAKASHI (JP)
KOBAYASHI TAKASHI (JP)
Application Number:
PCT/JP1994/001124
Publication Date:
February 09, 1995
Filing Date:
July 08, 1994
Export Citation:
Assignee:
GREEN CROSS CORP (JP)
MOCHIZUKI SHINOBU (JP)
KOBAYASHI TAKASHI (JP)
MOCHIZUKI SHINOBU (JP)
KOBAYASHI TAKASHI (JP)
International Classes:
C12N9/68; A61K38/00; (IPC1-7): C07K14/745; C07K1/18; A61K37/47
Foreign References:
| JPH0297386A | 1990-04-09 |
Other References:
BIOCHIMICA ET BIOPHYSICA ACTA, Vol. 221 (1970), PER WALLEN and BJORN WIMAN, "Characterization of Human Plasminogen", pages 20-30.
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