Title:
METHOD FOR PURIFYING POLYPEPTIDE
Document Type and Number:
WIPO Patent Application WO/2020/045477
Kind Code:
A1
Abstract:
A method for purifying a polypeptide is provided by which undesired polypeptides can be efficiently removed. The method is for purifying a desired polypeptide by subjecting a sample containing the desired polypeptide and undesired polypeptides to affinity chromatography, and includes a step in which the undesired polypeptides are treated with a treatment agent comprising a reducing agent.
Inventors:
DOI RYOU (JP)
MATSUDA TAKASHI (JP)
MATSUDA TAKASHI (JP)
Application Number:
PCT/JP2019/033649
Publication Date:
March 05, 2020
Filing Date:
August 28, 2019
Export Citation:
Assignee:
JSR CORP (JP)
JSR MICRO INC (US)
JSR MICRO N V (BE)
JSR MICRO INC (US)
JSR MICRO N V (BE)
International Classes:
C07K1/22; C07K16/00; C07K17/00; C07K19/00
Domestic Patent References:
| WO2015199196A1 | 2015-12-30 |
Foreign References:
| JP2016501898A | 2016-01-21 | |||
| US20120269856A1 | 2012-10-25 | |||
| JP2017531027A | 2017-10-19 |
Other References:
FEDULOVA N. ET AL.: "Expression and purification of catalytically active human PHD3 in Escherichia coli", PROTEIN EXPRESSION AND PURIFICATION, vol. 54, 2007, pages 1 - 10, XP022056628, DOI: 10.1016/j.pep.2007.02.018
PULLELA PK . ET AL.: "Fluorescence-based detection of thiols in vitro and in vivo using dithiol probes", ANALYTICAL BIOCHEMISTRY, vol. 352, 2006, pages 265 - 273, XP024942227, DOI: 10.1016/j.ab.2006.01.047
PULLELA PK . ET AL.: "Fluorescence-based detection of thiols in vitro and in vivo using dithiol probes", ANALYTICAL BIOCHEMISTRY, vol. 352, 2006, pages 265 - 273, XP024942227, DOI: 10.1016/j.ab.2006.01.047
Attorney, Agent or Firm:
THE PATENT CORPORATE BODY ARUGA PATENT OFFICE (JP)
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