Title:
METHOD FOR PURIFYING RECOMBINANT PROTEIN
Document Type and Number:
WIPO Patent Application WO/2018/066558
Kind Code:
A1
Abstract:
Disclosed is a method for purifying a recombinant protein from a recombinant cell in which a target recombinant protein is expressed within the cell as an insoluble body, said method characterized in that said recombinant cell is treated under conditions in which a protein derived from a host cell would dissolve, but said recombinant protein would not dissolve, in a first aprotic polar solvent to which an inorganic salt has been added or not added, a first soluble fraction is removed, and then an obtained first insoluble fraction is treated under conditions in which the recombinant protein would dissolve in a second aprotic polar solvent to which an inorganic salt has been added.
Inventors:
HONMA TOSHIMASA (JP)
Application Number:
PCT/JP2017/035983
Publication Date:
April 12, 2018
Filing Date:
October 03, 2017
Export Citation:
Assignee:
SPIBER INC (JP)
International Classes:
C07K1/14; C07K1/30; C07K1/36; C07K14/435; C12P21/02; D01F6/68
Domestic Patent References:
| WO2014103846A1 | 2014-07-03 | |||
| WO2014103799A1 | 2014-07-03 | |||
| WO2014103847A1 | 2014-07-03 | |||
| WO2014103847A1 | 2014-07-03 | |||
| WO2014103846A1 | 2014-07-03 |
Foreign References:
| JP2013523665A | 2013-06-17 | |||
| JP2008506409A | 2008-03-06 | |||
| JP2013523665A | 2013-06-17 | |||
| JP2004503204A | 2004-02-05 | |||
| JPS63248394A | 1988-10-14 | |||
| JPS58110600A | 1983-07-01 | |||
| JPS60221091A | 1985-11-05 | |||
| US4686191A | 1987-08-11 | |||
| US4939094A | 1990-07-03 | |||
| US5160735A | 1992-11-03 | |||
| JPH04278091A | 1992-10-02 | |||
| JPH07170984A | 1995-07-11 | |||
| JPH06133782A | 1994-05-17 | |||
| JP2002238569A | 2002-08-27 | |||
| JPH02227075A | 1990-09-10 | |||
| JP5584932B2 | 2014-09-10 |
Other References:
"NCBI Genbank", Database accession no. CAA56335.1
"NCBI Genbank", Database accession no. NP 611157.1
"NCBI Genbank", Database accession no. AAC98395
"NCBI Genbank", Database accession no. 147076
"NCBI Genbank", Database accession no. NP786966
PROC. NATL. ACAD. SCI. USA, vol. 69, 1972, pages 2110
GENE, vol. 17, 1982, pages 107
MOLECULAR & GENERAL GENETICS, vol. 168, 1979, pages 111
TAKAHASHI ET AL., J. BACTERIOL., vol. 156, 1983, pages 1130 - 1134
TAKAGI ET AL., AGRIC. BIOL. CHEM., vol. 53, 1989, pages 3099 - 3100
OKAMOTO ET AL., BIOSCI. BIOTECHNOL. BIOCHEM., vol. 61, 1997, pages 202 - 203
AGRIC. BIOL. CHEM., vol. 48, 1984, pages 669
AGRIC. BIOL. CHEM., vol. 53, 1989, pages 277
PROC. NATL. ACAD. SCI. USA, vol. 82, 1985, pages 4306
J. BACTERIOL., vol. 172, 1990, pages 2392
J. BACTERIOL., 1987, pages 1239 - 1245
SHIGEZOU UDAGA, JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY AND AGROCHEMISTRY JOURNAL, vol. 61, 1987, pages 669 - 676
APPL. MICROBIOL. BIOTECHNOL., vol. 30, 1989, pages 75 - 80
J. BIOCHEM., vol. 112, 1992, pages 488 - 491
APPL. ENVIRON. MICROBIOL., vol. 58, 1992, pages 525 - 531
J. BACTERIOL., vol. 177, 1995, pages 745 - 749
APPL. MICROBIOL. BIOTECHNOL., vol. 42, 1994, pages 358 - 363
MYERS, A. M. ET AL., GENE, vol. 45, 1986, pages 299 - 310
METHODS ENZYMOL., vol. 194, 1990, pages 182
PROC. NATL. ACAD. SCI., USA, vol. 81, 1984, pages 4889
J. BACTERIOL., vol. 153, 1983, pages 163
PROC. NATL. ACAD. SCI. USA, vol. 75, 1978, pages 1929
COHEN ET AL.: "Calcium chloride method", PROC. NATL. ACAD. SCI. USA, vol. 69, 1972, pages 2110
MOL. GEN. GENET., vol. 168, 1979, pages 111
J. MOL. BIOL., vol. 56, 1971, pages 209
"Baculovirus Expression Vectors, A Laboratory Manual", 1992, W. H. FREEMAN AND COMPANY
"Current Protocols in Molecular Biology, Baculovirus Expression Vectors, A Laboratory Manual", 1992, W H. FREEMAN AND COMPANY
BIO/TECHNOLOGY, vol. 6, 1988, pages 47
PROC. NATL. ACAD. SCI. USA, vol. 84, 1987, pages 7413
BIOSCI. BIOTECHNOL. BIOCHEM., vol. 59, 1995, pages 1795 - 1797
ENZYME MICROBIOL TECHNOL, vol. 6, 1984, pages 386 - 389
GENE, vol. 84, 1989, pages 329 - 334
BIOSCI BIOTECHNOL BIOCHEM, vol. 64, 2000, pages 1416 - 1421
BIOCHEM BIOPHYS RES COMMUN, vol. 112, 1983, pages 284 - 289
GENE, vol. 26, 1983, pages 205 - 221
MOL GEN GENET, vol. 261, 1999, pages 290 - 296
PROC NATL ACAD SCI USA, vol. 83, 1986, pages 4869 - 4873
GENE, vol. 57, 1987, pages 21 - 26
NATURE, vol. 195, 1962, pages 788
See also references of EP 3521299A4
"NCBI Genbank", Database accession no. NP 611157.1
"NCBI Genbank", Database accession no. AAC98395
"NCBI Genbank", Database accession no. 147076
"NCBI Genbank", Database accession no. NP786966
PROC. NATL. ACAD. SCI. USA, vol. 69, 1972, pages 2110
GENE, vol. 17, 1982, pages 107
MOLECULAR & GENERAL GENETICS, vol. 168, 1979, pages 111
TAKAHASHI ET AL., J. BACTERIOL., vol. 156, 1983, pages 1130 - 1134
TAKAGI ET AL., AGRIC. BIOL. CHEM., vol. 53, 1989, pages 3099 - 3100
OKAMOTO ET AL., BIOSCI. BIOTECHNOL. BIOCHEM., vol. 61, 1997, pages 202 - 203
AGRIC. BIOL. CHEM., vol. 48, 1984, pages 669
AGRIC. BIOL. CHEM., vol. 53, 1989, pages 277
PROC. NATL. ACAD. SCI. USA, vol. 82, 1985, pages 4306
J. BACTERIOL., vol. 172, 1990, pages 2392
J. BACTERIOL., 1987, pages 1239 - 1245
SHIGEZOU UDAGA, JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY AND AGROCHEMISTRY JOURNAL, vol. 61, 1987, pages 669 - 676
APPL. MICROBIOL. BIOTECHNOL., vol. 30, 1989, pages 75 - 80
J. BIOCHEM., vol. 112, 1992, pages 488 - 491
APPL. ENVIRON. MICROBIOL., vol. 58, 1992, pages 525 - 531
J. BACTERIOL., vol. 177, 1995, pages 745 - 749
APPL. MICROBIOL. BIOTECHNOL., vol. 42, 1994, pages 358 - 363
MYERS, A. M. ET AL., GENE, vol. 45, 1986, pages 299 - 310
METHODS ENZYMOL., vol. 194, 1990, pages 182
PROC. NATL. ACAD. SCI., USA, vol. 81, 1984, pages 4889
J. BACTERIOL., vol. 153, 1983, pages 163
PROC. NATL. ACAD. SCI. USA, vol. 75, 1978, pages 1929
COHEN ET AL.: "Calcium chloride method", PROC. NATL. ACAD. SCI. USA, vol. 69, 1972, pages 2110
MOL. GEN. GENET., vol. 168, 1979, pages 111
J. MOL. BIOL., vol. 56, 1971, pages 209
"Baculovirus Expression Vectors, A Laboratory Manual", 1992, W. H. FREEMAN AND COMPANY
"Current Protocols in Molecular Biology, Baculovirus Expression Vectors, A Laboratory Manual", 1992, W H. FREEMAN AND COMPANY
BIO/TECHNOLOGY, vol. 6, 1988, pages 47
PROC. NATL. ACAD. SCI. USA, vol. 84, 1987, pages 7413
BIOSCI. BIOTECHNOL. BIOCHEM., vol. 59, 1995, pages 1795 - 1797
ENZYME MICROBIOL TECHNOL, vol. 6, 1984, pages 386 - 389
GENE, vol. 84, 1989, pages 329 - 334
BIOSCI BIOTECHNOL BIOCHEM, vol. 64, 2000, pages 1416 - 1421
BIOCHEM BIOPHYS RES COMMUN, vol. 112, 1983, pages 284 - 289
GENE, vol. 26, 1983, pages 205 - 221
MOL GEN GENET, vol. 261, 1999, pages 290 - 296
PROC NATL ACAD SCI USA, vol. 83, 1986, pages 4869 - 4873
GENE, vol. 57, 1987, pages 21 - 26
NATURE, vol. 195, 1962, pages 788
See also references of EP 3521299A4
Attorney, Agent or Firm:
HASEGAWA Yoshiki et al. (JP)
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