Title:
METHOD FOR PURIFYING REVERSIBLY BLOCKED DEOXYRIBONUCLEOTIDE TRIPHOSPHATE AND SEQUENCING METHOD
Document Type and Number:
WIPO Patent Application WO/2018/107350
Kind Code:
A1
Abstract:
Disclosed in the present invention are a method for purifying a reversibly blocked deoxyribonucleotide triphosphate and a sequencing method, the method for purifying a reversibly blocked deoxyribonucleotide triphosphate comprising: using an adenylate cyclase to treat a 3' reversibly blocked dATP, and/or using guanylate cyclase to treat a 3' reversibly blocked dGTP. When used in sequencing, a reversibly blocked dNTP which has been treated may lead to lower phase shifting, especially for a pre-determined phase, so as to improve sequencing quality.
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Inventors:
LIU ERKAI (CN)
XU CHONGJUN (CN)
ZHANG WENWEI (CN)
XU CHONGJUN (CN)
ZHANG WENWEI (CN)
Application Number:
PCT/CN2016/109597
Publication Date:
June 21, 2018
Filing Date:
December 13, 2016
Export Citation:
Assignee:
BGI SHENZHEN (CN)
International Classes:
C12Q1/68
Domestic Patent References:
| WO2014139596A1 | 2014-09-18 |
Foreign References:
| CN101942000A | 2011-01-12 |
Other References:
TANG, DAONIAN ET AL.: "Synthesis and Application of Four Fluorescence Labeled Nucleotides Through Disulfide as Reversible Terminators in DNA Sequencing by Synthesis", CHEMICAL JOURNAL OF CHINESE UNIVERSITIES, vol. 35, 30 November 2014 (2014-11-30), pages 2346 - 2352, XP055299457
Attorney, Agent or Firm:
DHC IP ATTORNEYS (CN)
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