Title:
METHOD FOR PURIFYING THROMBIN SUBSTRATES AND/OR INHIBITORS OR METHOD FOR ELIMINATING THE SAME
Document Type and Number:
WIPO Patent Application WO/1999/020655
Kind Code:
A1
Abstract:
A method for purifying thrombin substrates and/or inhibitors characterized by involving the step of effecting affinity chromatography with the use of an affinity adsorbent having thrombin or its analogue from which the activity (nucleophilicity) has been taken off without inducing any steric hindrance in binding, as a ligand, to the substrates and/or inhibitors. This method aims at efficiently, safely and stably purifying inexpensive and highly pure thrombin substrates and/or inhibitors by using a novel and inexpensive separation/purification means with the use of a convenient separation/purification procedure and a small number of purification/separation steps. By using the above method, blood coagulation factors VIII and XIII, protein C, etc. can be purified at a high purity in a high yield as compared with the conventional purification methods.
Inventors:
SUZUKI TOYOAKI (JP)
HOSOKAWA KAZUYA (JP)
NAGATA MASANORI (JP)
HOSOKAWA KAZUYA (JP)
NAGATA MASANORI (JP)
Application Number:
PCT/JP1998/004812
Publication Date:
April 29, 1999
Filing Date:
October 23, 1998
Export Citation:
Assignee:
FUJIMORI KOGYO CO (JP)
SUZUKI TOYOAKI (JP)
HOSOKAWA KAZUYA (JP)
NAGATA MASANORI (JP)
SUZUKI TOYOAKI (JP)
HOSOKAWA KAZUYA (JP)
NAGATA MASANORI (JP)
International Classes:
C07K14/755; C12N9/10; C12N9/64; C12N9/74; C12N11/00; (IPC1-7): C07K14/745; C07K1/22; A61K38/43
Foreign References:
| JPS5810522A | 1983-01-21 | |||
| JPS59167519A | 1984-09-21 | |||
| JPH1059863A | 1998-03-03 | |||
| EP0882789A2 | 1998-12-09 |
Other References:
TOMONO T., SAWADA E.: "PREPARATION OF ANHYDRO-THROMBIN AND ITS INTERACTION WITH PLASMA ANTITHROMBIN III.", ACTA HAEMATOLOGICA, S. KARGER, BASEL, CH, vol. 49., no. 04., 1 July 1986 (1986-07-01), CH, pages 969 - 979., XP000912136, ISSN: 0001-5792
ASHTON R. W., ET AL.: "PREPARATION AND CHARACTERIZATION OF ANHYDROTHROMBIN.", BIOCHEMISTRY., MAIK NAUKA - INTERPERIODICA, RU, vol. 34., no. 19., 1 January 1995 (1995-01-01), RU, pages 6454 - 6463., XP000938937, ISSN: 0006-2979, DOI: 10.1021/bi00019a027
See also references of EP 1026172A4
ASHTON R. W., ET AL.: "PREPARATION AND CHARACTERIZATION OF ANHYDROTHROMBIN.", BIOCHEMISTRY., MAIK NAUKA - INTERPERIODICA, RU, vol. 34., no. 19., 1 January 1995 (1995-01-01), RU, pages 6454 - 6463., XP000938937, ISSN: 0006-2979, DOI: 10.1021/bi00019a027
See also references of EP 1026172A4
Attorney, Agent or Firm:
Hatta, Mikio (Nibancho Chiyoda-ku Tokyo, JP)
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