Title:
METHOD FOR PURIFYING VIRUS
Document Type and Number:
WIPO Patent Application WO2004112707
Kind Code:
A3
Abstract:
A process for the purification of viruses from a cell lysate preparation is described, consisting of preferably two successive chromatographic steps; the first a clarification step utilizing size exclusion chromatography, and the second, a virus capture and release step using anion exchange chromatography, which successive chromatographic steps have the advantage of purifying virus, and avoiding chromatography buffer conductivity adjustments.
Inventors:
KOSTEL PAUL (US)
YANAGIMACHI KURT (US)
OLSON CHARLES (US)
YANAGIMACHI KURT (US)
OLSON CHARLES (US)
Application Number:
PCT/US2004/019126
Publication Date:
March 02, 2006
Filing Date:
June 14, 2004
Export Citation:
Assignee:
ONYX PHARMA INC (US)
KOSTEL PAUL (US)
KOSTEL PAUL (US)
International Classes:
C12N7/02; A61K; (IPC1-7): C12N7/02
Foreign References:
| US6143548A | 2000-11-07 |
Other References:
HUYGHE B.G. ET AL: "Purification of a type 5 recombinant adenovirus encoding human p53 by column chromatography", HUMAN GENE THERAPY, vol. 6, no. 11, 1 November 1995 (1995-11-01), pages 1403 - 1416, XP000196636
See also references of EP 1633321A4
See also references of EP 1633321A4
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