Title:
METHOD FOR QUANTIFYING 3,6-ANHYDRO-L-GALACTOSE USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND DIFFERENTIAL REFRACTOMETRIC DETECTOR
Document Type and Number:
WIPO Patent Application WO/2019/013489
Kind Code:
A3
Abstract:
The present invention relates to a method for quantifying 3,6-anhydro-L-galactose using high-performance liquid chromatography and a differential refractometric detector, whereby L-AHG and D-galactose, which are saccharification products of agarose or agar, can be independently quantified by controlling the separation conditions of the high-performance liquid chromatography/differential refractometric detector, even though the two materials are difficult to separate due to having similar molecular weights and structures.
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Inventors:
KIM KYOUNG HEON (KR)
CHO KYUNG-MUN (KR)
YUN EUN-JU (KR)
KIM DONG-HYUN (KR)
CHO KYUNG-MUN (KR)
YUN EUN-JU (KR)
KIM DONG-HYUN (KR)
Application Number:
PCT/KR2018/007612
Publication Date:
March 28, 2019
Filing Date:
July 05, 2018
Export Citation:
Assignee:
UNIV KOREA RES & BUS FOUND (KR)
International Classes:
G01N30/62; G01N30/02; G01N30/86; G01N30/88
Foreign References:
KR101569278B1 | 2015-11-16 | |||
KR101031896B1 | 2011-05-02 | |||
KR20040066336A | 2004-07-27 |
Other References:
LEE, CHAN HYOUNG ET AL.: "Sccharification of Agar Using Hydrothermal Pretreatment and Enzymes Supplemented with Agarolylic beta-galactosidase", PROCESS BIOC HEMISTRY, vol. 50, 2015, pages 1629 - 1633
CHAVEZ-SERVIN, JORGE L. ET AL.: "Analysis of Mono- and Disaccharides in Milk-based Formulae by High-performance Liquid Chromatography with Refractive Index Detection", JOURNAL OF CHROMATOGRAPHY A, vol. 1043, 2004, pages 211 - 215, XP004520521, DOI: doi:10.1016/j.chroma.2004.06.002
CHAVEZ-SERVIN, JORGE L. ET AL.: "Analysis of Mono- and Disaccharides in Milk-based Formulae by High-performance Liquid Chromatography with Refractive Index Detection", JOURNAL OF CHROMATOGRAPHY A, vol. 1043, 2004, pages 211 - 215, XP004520521, DOI: doi:10.1016/j.chroma.2004.06.002
Attorney, Agent or Firm:
DANA PATENT LAW FIRM (KR)
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