Title:
METHOD OF QUANTIFYING OXIDIZED PROTEIN, LABELING REAGENTS FOR QUANTIFYING OXIDIZED PROTEIN AND LABELING REAGENT KIT FOR QUANTIFYING OXIDIZED PROTEIN
Document Type and Number:
WIPO Patent Application WO/2007/111193
Kind Code:
A1
Abstract:
An oxidized protein, which has been denatured by oxidation, is labeled with labeling reagents and quantified by mass spectrometry. In this process, a first labeling reagent, which is capable of reacting with the oxidized protein, and a second labeling reagent, which has the same chemical structure as the first labeling reagent and in which at least a part of the constituting atoms is substituted by an isotope of the atom concerned, are employed as the labeling agents. The oxidized protein labeled with the first labeling reagent and the oxidized protein labeled with the second labeling reagent are mixed together at various mixing ratios and subjected to mass spectrometry. As the labeling reagents, use is made of, for example, 2,4-dinitrophenylhydrazine and 2,4-dinitrophenylhydrazine in which a carbon atom in the phenyl group has been substituted by a stable isotope (13C).
Inventors:
TSUJIMOTO KAZUO (JP)
HAYASHI AKIO (JP)
KAWAI TAKAAKI (JP)
MATSUMOTO HIROYUKI (JP)
HAYASHI AKIO (JP)
KAWAI TAKAAKI (JP)
MATSUMOTO HIROYUKI (JP)
Application Number:
PCT/JP2007/055617
Publication Date:
October 04, 2007
Filing Date:
March 20, 2007
Export Citation:
Assignee:
JAPAN ADV INST SCIENCE & TECH (JP)
TSUJIMOTO KAZUO (JP)
HAYASHI AKIO (JP)
KAWAI TAKAAKI (JP)
MATSUMOTO HIROYUKI (JP)
TSUJIMOTO KAZUO (JP)
HAYASHI AKIO (JP)
KAWAI TAKAAKI (JP)
MATSUMOTO HIROYUKI (JP)
International Classes:
G01N27/62
Domestic Patent References:
| WO2006039456A1 | 2006-04-13 | |||
| WO2003028658A2 | 2003-04-10 |
Foreign References:
| JP2005315688A | 2005-11-10 | |||
| JP2005300430A | 2005-10-27 |
Other References:
ZUREK G. ET AL.: "2,4-Dinitro-3,5,6-trideuterophenylhydrazones for the quantitiation of aldehydes and ketones in air samples by liquid chromatography-mass spectrometry", JOURNAL OF CHROMATOGRAPHY, vol. 869, no. 1/2, 11 February 2000 (2000-02-11), pages 251 - 259, XP004186569
KÖLLIKER S. ET AL.: "Unusual MSn fragmentation patterns of 2,4-dinitrio-phenylhydrazone and its propanone derivative", RAPID COMMUNICATIONS IN MASS SPECTROMETRY, vol. 15, no. 22, 2001, pages 2117 - 2126, XP003018120
DZELETOVIC S. ET AL.: "Determination of Cholesterol Oxidation Products in Human Plasma by Isotope Dilution-Mass Spectrometry", ANALYTICAL CHEMISTRY, vol. 225, no. 1, 1995, pages 73 - 80, XP003018121
KÖLLIKER S. ET AL.: "Unusual MSn fragmentation patterns of 2,4-dinitrio-phenylhydrazone and its propanone derivative", RAPID COMMUNICATIONS IN MASS SPECTROMETRY, vol. 15, no. 22, 2001, pages 2117 - 2126, XP003018120
DZELETOVIC S. ET AL.: "Determination of Cholesterol Oxidation Products in Human Plasma by Isotope Dilution-Mass Spectrometry", ANALYTICAL CHEMISTRY, vol. 225, no. 1, 1995, pages 73 - 80, XP003018121
Attorney, Agent or Firm:
KIMORI, Yuhei et al. (Room 205Nishimura Bldg.,4-4-25, Sainen, Kanazawa-shi, Ishikawa 24, JP)
Download PDF:
Previous Patent: ORGANIC ELECTROLUMINESCENT ELEMENT, AND ORGANIC ELECTROLUMINESCENT DISPLAY
Next Patent: PAPER FASTENER AND FILE OR FOLDER
Next Patent: PAPER FASTENER AND FILE OR FOLDER