Title:
METHOD FOR RAPID DETECTION OF SPORES BY FLUORESCENT STAINING
Document Type and Number:
WIPO Patent Application WO/2015/037578
Kind Code:
A1
Abstract:
The present invention provides a method, etc., for detecting spores, characterized in that a sample is stained by a fluorescent stain that stains spores but does not stain vegetative cells, and the fluorescence is observed.
Inventors:
TAKAMATSU HIROMU (JP)
KUWANA RITSUKO (JP)
FUJITA YASUHIRO (JP)
KINOUCHI TOMOYUKI (JP)
KUWANA RITSUKO (JP)
FUJITA YASUHIRO (JP)
KINOUCHI TOMOYUKI (JP)
Application Number:
PCT/JP2014/073792
Publication Date:
March 19, 2015
Filing Date:
September 09, 2014
Export Citation:
Assignee:
JOSHO GAKUEN EDUCATIONAL FOUNDATION (JP)
International Classes:
C12Q1/04; C12N1/20; G01N33/48
Foreign References:
| JP2000210099A | 2000-08-02 | |||
| JP2011239749A | 2011-12-01 |
Other References:
TERUO ITOI ET AL.: "Fushin Hakushoku Funmatsu kara no Saikin Kenshutsu no Tameno Gram Senshoku, Gaho Senshoku Kensaho no Yuyosei", REPORTS OF NATIONAL RESEARCH INSTITUTE OF POLICE SCIENCE , RESEARCH ON FORENSIC SCIENCE, vol. 56, no. 1, 2006, pages 13 - 18
SCHAEFFER A. B. ET AL.: "SCIENTIFIC APPARATUS AND LABORATORY METHODS, A SIMPLIFIED METHOD OF STAINING ENDOSPORES", SCIENCE, vol. 77, 1933, pages 194
ARSECULERATNE S. N. ET AL.: "The assessment of the viability of the endospores of Rhinosporidium seeberi with MTT (3-[4,5- dimethyl-2-thiazolyl]-2, 5-diphenyl-2H- tetrazolium bromide", MYCOLOGICAL RESEARCH, vol. 108, no. 12, 2004, pages 1423 - 1430, XP022443488, DOI: doi:10.1017/S0953756204001595
YONEMOTO Y. ET AL.: "Resistance of Yeast and Bacterial Spores to High Voltage Electric Pulses", JOURNAL OF FERMENTATION AND BIOENGINEERING, vol. 75, no. 2, 1993, pages 99 - 102, XP025752800, DOI: doi:10.1016/0922-338X(93)90217-V
STRUGGER S.: "Der gegenwartige stand der forschung auf dem gebiet der fluoreszenzmikroskopischen untersuchung der BAKTERIEN", MIKROSKOPIE, vol. 3, no. 1-2, 1948, pages 23 - 38
DHOLPURIA S.: "Production and Purification of alkali stable xylanase from Bacillus sp.", INT. J. CURR. MICROBIOL. APP. SCI, vol. 3, no. 3, March 2014 (2014-03-01), pages 365 - 377
SCHAEFFER A. B. ET AL.: "SCIENTIFIC APPARATUS AND LABORATORY METHODS, A SIMPLIFIED METHOD OF STAINING ENDOSPORES", SCIENCE, vol. 77, 1933, pages 194
ARSECULERATNE S. N. ET AL.: "The assessment of the viability of the endospores of Rhinosporidium seeberi with MTT (3-[4,5- dimethyl-2-thiazolyl]-2, 5-diphenyl-2H- tetrazolium bromide", MYCOLOGICAL RESEARCH, vol. 108, no. 12, 2004, pages 1423 - 1430, XP022443488, DOI: doi:10.1017/S0953756204001595
YONEMOTO Y. ET AL.: "Resistance of Yeast and Bacterial Spores to High Voltage Electric Pulses", JOURNAL OF FERMENTATION AND BIOENGINEERING, vol. 75, no. 2, 1993, pages 99 - 102, XP025752800, DOI: doi:10.1016/0922-338X(93)90217-V
STRUGGER S.: "Der gegenwartige stand der forschung auf dem gebiet der fluoreszenzmikroskopischen untersuchung der BAKTERIEN", MIKROSKOPIE, vol. 3, no. 1-2, 1948, pages 23 - 38
DHOLPURIA S.: "Production and Purification of alkali stable xylanase from Bacillus sp.", INT. J. CURR. MICROBIOL. APP. SCI, vol. 3, no. 3, March 2014 (2014-03-01), pages 365 - 377
Attorney, Agent or Firm:
TAKASHIMA, HAJIME (JP)
Takashima 1 (JP)
Takashima 1 (JP)
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