Title:
METHOD FOR RAPIDLY DETECTING PLANT DISEASE-RESISTANT GENES
Document Type and Number:
WIPO Patent Application WO/2021/004098
Kind Code:
A1
Abstract:
Provided is a method for rapidly detecting plant disease-resistant genes, the method comprising the following steps: introducing disease-resistant genes into agrobacterium to obtain recombinant bacteria; infecting the back side of grape leaves with the recombinant bacteria, transforming the disease-resistant genes into the grape leaves, and inoculating the front side of the grape leaves with pathogenic bacteria to achieve the detection of the disease-resistant genes; introducing the disease-resistant genes into the agrobacterium through a recombinant vector, which is a vector obtained by cloning a target gene into the multiple cloning site of an expression vector. The method for rapidly detecting plant disease-resistant genes is suitable for the expression detection of rapidly and transiently transformed foreign genes of various transgenic plants.
Inventors:
ZHANG YING (CN)
LIU RUITAO (CN)
LIU CHONGHUAI (CN)
FAN XIUCAI (CN)
JIANG JIANFU (CN)
LIU RUITAO (CN)
LIU CHONGHUAI (CN)
FAN XIUCAI (CN)
JIANG JIANFU (CN)
Application Number:
PCT/CN2020/081955
Publication Date:
January 14, 2021
Filing Date:
March 29, 2020
Export Citation:
Assignee:
ZHENGZHOU FRUIT RES INSTITUTE CAAS (CN)
International Classes:
C12N15/82; A01H5/00; A01H6/88; G01N33/68
Foreign References:
| CN110408648A | 2019-11-05 | |||
| CN107501399A | 2017-12-22 | |||
| CN102121004A | 2011-07-13 |
Other References:
YANG LI-NA, LIU JIE ZHUANG ZHI-MIN , ZHANG ZHEN ZHOU BEI-BEI ,TAO JIAN-MIN: "Studies on Agrobacterium-mediated transformation of ’Manicure Finger’ grape with Barnase gene", JOURNAL OF NANJING AGRICULTURAL UNIVERSITY, vol. 32, no. 1, 30 January 2009 (2009-01-30), pages 31 - 35, XP055772668, ISSN: 1000-2030
MARCHIVE CHLOÉ, LÉON CÉLINE, KAPPEL CHRISTIAN, COUTOS-THÉVENOT PIERRE, CORIO-COSTET MARIE-FRANCE, DELROT SERGE, LAUVERGEAT VIRGINI: "Over-Expression of VvWRKY1 in Grapevines Induces Expression of Jasmonic Acid Pathway-Related Genes and Confers Higher Tolerance to the Downy Mildew", PLOS ONE, vol. 8, no. 1, e54185, 14 January 2013 (2013-01-14), pages 1 - 8, XP055772669, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0054185
MARCHIVE CHLOÉ, LÉON CÉLINE, KAPPEL CHRISTIAN, COUTOS-THÉVENOT PIERRE, CORIO-COSTET MARIE-FRANCE, DELROT SERGE, LAUVERGEAT VIRGINI: "Over-Expression of VvWRKY1 in Grapevines Induces Expression of Jasmonic Acid Pathway-Related Genes and Confers Higher Tolerance to the Downy Mildew", PLOS ONE, vol. 8, no. 1, e54185, 14 January 2013 (2013-01-14), pages 1 - 8, XP055772669, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0054185
Attorney, Agent or Firm:
SCIHEAD IP LAW FIRM (CN)
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