Title:
METHOD FOR RAPIDLY DETERMINING NUMBER OF BACTERIA AND EQUIPMENT FOR DETERMINING NUMBER OF BACTERIA
Document Type and Number:
WIPO Patent Application WO/1996/030542
Kind Code:
A1
Abstract:
The inventions as set forth in claims 1 to 9 provide methods and equipment for determining the number of bacteria in a sample rapidly and readily in a single filtration operation without necessitating any special facility and special knowledge. The inventions as set forth in claims 10 to 18 provide methods and kits for similarly determining the number of bacteria in a sample rapidly and readily in a single-stage filtration operation using an injection syringe without using any sampling tool such as a dropping pipette. The invention as set forth in claim 1 provides a method for rapidly determining the number of bacteria in a sample characterized by leading a bacterial sample into a tubular filtration vessel which has in its interior a hydrophobic filter for detecting bacteria, a dye solution composition disposed on such a side of the filter as will receive a bacterial sample, and a filter support (and further a piston and an aqueous solution in claim 2) disposed on the opposite side of the filter from the bacterial sample reception side to stain the sample, filtering the stained sample by suction from the support side to simultaneously conduct the trapping of the stained bacteria on the filter and the removal of excess dye, and determining the degree of coloring of the filter. The invention as set forth in claim 10 provides a method for rapidly determining the number of bacteria in a sample characterized by leading a bacterial sample by suction into a filtration vessel having in its interior a hydrophobic filter for detecting bacteria, pushing the sample into a dye solution composition, trapping the stained bacteria on the hydrophobic filter by filtration under suction, and determining the degree of coloring of the filter.
Inventors:
SATO MIKIO (JP)
ITO TOMOMI (JP)
ITO TOMOMI (JP)
Application Number:
PCT/JP1996/000815
Publication Date:
October 03, 1996
Filing Date:
March 28, 1996
Export Citation:
Assignee:
IDEMITSU KOSAN CO (JP)
SATO MIKIO (JP)
ITO TOMOMI (JP)
SATO MIKIO (JP)
ITO TOMOMI (JP)
International Classes:
C12M1/34; C12Q1/06; (IPC1-7): C12Q1/06; C12M1/34
Foreign References:
| JPH04218392A | 1992-08-07 |
Other References:
EDITED BY KYOTO UNIVERSITY ROKUSEIKAI, "New Edit. Agricultural Chemistry Experiment Book (Vol. 2)", 10 April 1957, SANGYO TOSHO (TOKYO), p. 428-429.
See also references of EP 0818540A4
See also references of EP 0818540A4
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