Login| Sign Up| Help| Contact|

Patent Searching and Data


Title:
METHOD AND REAGENT FOR GENE SEQUENCE ANALYSIS
Document Type and Number:
WIPO Patent Application WO/2011/019031
Kind Code:
A1
Abstract:
Disclosed is a nucleic acid which has nucleic acid substrate characteristics equivalent to those of dATP, has a low specificity for luciferase, exerts no undesirable effect on enzymatic reactions such as synthesis of a complementary chain and, therefore, is particularly suitable for the pyrosequencing method. As a nucleic acid substrate complementary to base T, a 7-substituted deoxyribonucleotide triphosphate, which is modified with a substituent at the 7-position of the purine group, is used as a substitute for a nucleotide α-thiotriphosphate analog.

Inventors:
KUWAHARA Masayasu (5-1 Tenjin-cho 1-chome, Kiryu-sh, Gunma 15, 〒3768515, JP)
▲桑▼原正靖 (〒15 群馬県桐生市天神町一丁目5番1号 国立大学法人群馬大学内 Gunma, 〒3768515, JP)
KAJIYAMA Tomoharu (HITACHI LTD., 280, Higashikoigakubo 1-chome, Kokubunji-sh, Tokyo 01, 〒1858601, JP)
Application Number:
JP2010/063529
Publication Date:
February 17, 2011
Filing Date:
August 10, 2010
Export Citation:
Click for automatic bibliography generation   Help
Assignee:
HITACHI, LTD. (6-6 Marunouchi 1-chome, Chiyoda-ku Tokyo, 80, 〒1008280, JP)
株式会社日立製作所 (〒80 東京都千代田区丸の内一丁目6番6号 Tokyo, 〒1008280, JP)
NATIONAL UNIVERSITY CORPORATION GUNMA UNIVERSITY (2 Aramaki-machi 4-chome, Maebashi-shi Gunma, 10, 〒3718510, JP)
国立大学法人群馬大学 (〒10 群馬県前橋市荒牧町四丁目2番地 Gunma, 〒3718510, JP)
KUWAHARA Masayasu (5-1 Tenjin-cho 1-chome, Kiryu-sh, Gunma 15, 〒3768515, JP)
International Classes:
C07H19/20; C12Q1/68
Domestic Patent References:
WO2008144315A1
WO1998013523A1
Foreign References:
JP2007056001A
Other References:
KUWAHARA MASAYASU ET AL.: 'Substrate property and incorporation accuracy of various dATP analogs during enzymatic polymerization using thermostable DNA polymerases' NUCLEIC ACIDS SYMPOSIUM SERIES no. 50, 2006, pages 31 - 32
ERIKSSON J. ET AL.: '7-deaza-2'-deoxyadenosine- 5'-triphosphate as an alternative nucleotide for the pyrosequencing technology' NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS vol. 23, no. 10, 2004, pages 1583 - 1594
Attorney, Agent or Firm:
HIRAKI Yusuke (Kamiya-cho MT Bldg. 19F, 3-20 Toranomon 4-chome, Minato-k, Tokyo 01, 〒1050001, JP)
Download PDF: