Title:
METHOD AND REAGENT FOR GENE SEQUENCE ANALYSIS
Document Type and Number:
WIPO Patent Application WO/2011/019031
Kind Code:
A1
Abstract:
Disclosed is a nucleic acid which has nucleic acid substrate characteristics equivalent to those of dATP, has a low specificity for luciferase, exerts no undesirable effect on enzymatic reactions such as synthesis of a complementary chain and, therefore, is particularly suitable for the pyrosequencing method.
As a nucleic acid substrate complementary to base T, a 7-substituted deoxyribonucleotide triphosphate, which is modified with a substituent at the 7-position of the purine group, is used as a substitute for a nucleotide α-thiotriphosphate analog.
Inventors:
KUWAHARA MASAYASU (JP)
KAJIYAMA TOMOHARU (JP)
KAMBARA HIDEKI (JP)
KAJIYAMA TOMOHARU (JP)
KAMBARA HIDEKI (JP)
Application Number:
PCT/JP2010/063529
Publication Date:
February 17, 2011
Filing Date:
August 10, 2010
Export Citation:
Assignee:
HITACHI LTD (JP)
UNIV GUNMA NAT UNIV CORP (JP)
KUWAHARA MASAYASU (JP)
KAJIYAMA TOMOHARU (JP)
KAMBARA HIDEKI (JP)
UNIV GUNMA NAT UNIV CORP (JP)
KUWAHARA MASAYASU (JP)
KAJIYAMA TOMOHARU (JP)
KAMBARA HIDEKI (JP)
International Classes:
C07H19/20; C12Q1/68
Domestic Patent References:
| WO2008144315A1 | 2008-11-27 | |||
| WO1998013523A1 | 1998-04-02 |
Foreign References:
| JP2007056001A | 2007-03-08 |
Other References:
KUWAHARA MASAYASU ET AL.: "Substrate property and incorporation accuracy of various dATP analogs during enzymatic polymerization using thermostable DNA polymerases", NUCLEIC ACIDS SYMPOSIUM SERIES, no. 50, 2006, pages 31 - 32
ERIKSSON J. ET AL.: "7-deaza-2'-deoxyadenosine- 5'-triphosphate as an alternative nucleotide for the pyrosequencing technology", NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, vol. 23, no. 10, 2004, pages 1583 - 1594
ERIKSSON J. ET AL.: "7-deaza-2'-deoxyadenosine- 5'-triphosphate as an alternative nucleotide for the pyrosequencing technology", NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, vol. 23, no. 10, 2004, pages 1583 - 1594
Attorney, Agent or Firm:
HIRAKI YUSUKE (JP)
Yusuke Hiraki (JP)
Yusuke Hiraki (JP)
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