Title:
METHOD AND REAGENT FOR SEPARATION, ENRICHMENT AND NUCLEIC ACID EXTRACTION OF PATHOGENIC MICROORGANISMS IN BLOOD
Document Type and Number:
WIPO Patent Application WO/2022/206726
Kind Code:
A1
Abstract:
Provided are a method and a reagent for the separation, enrichment and nucleic acid extraction of pathogenic microorganisms in blood. The method comprises: firstly, adding a blood selective lysis solution A and a pathogenic microorganism settling aid to a blood sample to selectively lyse blood cells, and settling pathogenic microorganisms by means of high-speed centrifugation; then adding a blood selective lysis solution B to selectively lyse the blood sample again, and then adding a washing solution, and uniformly mixing and then washing same; and finally adding a nucleic acid extraction reagent, centrifuging and enriching the pathogenic microorganisms, and then performing lysis in combination with a physical method to obtain a pathogenic microorganism nucleic acid sample. The nucleic acid extraction method can quickly and accurately extract the nucleic acid of an infectious target pathogen, which can meet a molecular biology detection requirement, from a blood sample not greater than 10 mL, and the nucleic acid extraction step is simple, complex processing equipment is not needed, and the obtained nucleic acid can be used for various downstream detections in molecular biology.
Inventors:
XU XUEJIAO (CN)
HE CUITING (CN)
HE CUITING (CN)
Application Number:
PCT/CN2022/083559
Publication Date:
October 06, 2022
Filing Date:
March 29, 2022
Export Citation:
Assignee:
BEIJING YUANWEI BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12N15/10; C12N1/02
Domestic Patent References:
| WO2017129814A1 | 2017-08-03 |
Foreign References:
| CN113073096A | 2021-07-06 | |||
| US20170275612A1 | 2017-09-28 | |||
| CN110195096A | 2019-09-03 | |||
| US20160068897A1 | 2016-03-10 | |||
| CN108699550A | 2018-10-23 |
Other References:
ZHANG ZHONGFEN, TAN YA , XIA WUJIE , JIANG YUEHUA: "Improvement of Erythrocyte Lysis Method in PCR Technology", ZHONGGUO YOUSHENG YU YICHUAN ZAZHI- CHINA JOURNAL OF BIRTH HEALTH & HEREDITY, ZHONGGUO YOUSHENG KEXUE XIEHUI, CN, vol. 6, no. 5, 31 December 1998 (1998-12-31), CN , pages 27 - 27, XP055973133, ISSN: 1006-9534, DOI: 10.13404/j.cnki.cibhh.1998.05.016
Attorney, Agent or Firm:
BEIJING JUHONG INTELLECTUAL PROPERTY AGENCY (GP) (CN)
Download PDF:
Previous Patent: NOVEL COMPOUNDS USEFUL AS STING AGONISTS AND USES THEREOF
Next Patent: DRIVING REMINDER METHOD AND DEVICE
Next Patent: DRIVING REMINDER METHOD AND DEVICE