Title:
METHOD FOR RECOVERING EXTRACELLULAR VESICLES
Document Type and Number:
WIPO Patent Application WO/2017/154951
Kind Code:
A1
Abstract:
Provided is a method for recovering extracellular vesicles having excellent separation efficiency for separating and trapping the extracellular vesicles such as exosomes in a dispersion, the method furthermore making it possible to efficiently recover the trapped extracellular vesicles. A method for recovering extracellular vesicles in which a dispersion containing extracellular vesicles having an average particle size of 10-700 nm is brought into contact with a perforated substrate comprising a porous material, whereby extracellular vesicles from the dispersion are trapped on a perforated substrate and separated, and then the trapped extracellular vesicles are recovered from the perforated substrate.
Inventors:
BABA YOSHINOBU (JP)
YUKAWA HIROSHI (JP)
ONOSHIMA DAISUKE (JP)
NAKAMURA YUUTA (JP)
YAMAZAKI SHUJI (JP)
KOGUCHI RYOHEI (JP)
SUWA KUMIKO (JP)
YUKAWA HIROSHI (JP)
ONOSHIMA DAISUKE (JP)
NAKAMURA YUUTA (JP)
YAMAZAKI SHUJI (JP)
KOGUCHI RYOHEI (JP)
SUWA KUMIKO (JP)
Application Number:
PCT/JP2017/009150
Publication Date:
September 14, 2017
Filing Date:
March 08, 2017
Export Citation:
Assignee:
UNIV NAGOYA (JP)
ASAHI GLASS CO LTD (JP)
ASAHI GLASS CO LTD (JP)
International Classes:
C12N1/00; B03B5/00
Domestic Patent References:
| WO2015050891A2 | 2015-04-09 | |||
| WO2016136978A1 | 2016-09-01 |
Foreign References:
| JP2014219384A | 2014-11-20 | |||
| JPS62501678A | 1987-07-09 | |||
| JP2014519331A | 2014-08-14 | |||
| JPH08502200A | 1996-03-12 | |||
| JP2016188772A | 2016-11-04 |
Other References:
YUKAWA, H. ET AL.: "Analysis of exosomes derived from cancer cells by nanobiodevices", ORGAN BIOLOGY, vol. 22, no. 2, 2015, pages 193 - 198, XP055602554, ISSN: 2188-0204
NAKAMURA, Y. ET AL.: "Development of exosome extraction method by minute layer glass device", CSJ: THE CHEMICAL SOCIETY OF JAPAN DAI 96 SHUNKI NENKAI (2016) KOEN YOKOSHU, 2016
NAKAMURA, Y. ET AL.: "Development of exosome extraction method by minute layer glass device", CSJ: THE CHEMICAL SOCIETY OF JAPAN DAI 96 SHUNKI NENKAI (2016) KOEN YOKOSHU, 2016
Attorney, Agent or Firm:
SAKURA PATENT OFFICE, P.C. (JP)
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