Title:
METHOD FOR REGULATING NUMBER OF MAIZE TASSEL BRANCHES
Document Type and Number:
WIPO Patent Application WO/2024/060534
Kind Code:
A1
Abstract:
Provided is a method for regulating the number of maize tassel branches, comprising: constructing an miR529 overexpression vector with two tandem repeating hairpin structures by utilizing T-DNA insertion transgenic technology; transferring the miR529 overexpression vector into an immature embryo of maize inbred line C01 by means of an agrobacterium strain EHA105 to give a plurality of transgenic events; and verifying the plurality of transgenic events by means of PCR, and selecting an independent event with increased expression of miR529 in the tassel by means of Northern blotting. By using the overexpression transgenic technology to improve the expression of miR529, the present invention reduces the number of tassel branches and finally improves the yield per unit area in densely planted maize populations.
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Inventors:
LIU LIN (CN)
ZHANG RUI (CN)
SONG QIAOQIAO (CN)
ZHANG RUI (CN)
SONG QIAOQIAO (CN)
Application Number:
PCT/CN2023/081298
Publication Date:
March 28, 2024
Filing Date:
March 14, 2023
Export Citation:
Assignee:
UNIV SHENZHEN (CN)
International Classes:
C12Q1/6895; C12N15/11; C12N15/113; C12N15/82
Foreign References:
| CN106170548A | 2016-11-30 | |||
| CN115003816A | 2022-09-02 | |||
| CN109161558A | 2019-01-08 |
Other References:
WEI, HONGBIN ET AL.: "Exploiting SPL genes to improve maize plant architecture tailored for high-density planting", JOURNAL OF EXPERIMENTAL BOTANY, vol. 69, no. 20, 10 July 2018 (2018-07-10), pages 4675 - 4688, XP055853143, DOI: 10.1093/jxb/ery258
MOREA EDNA GICELA ORTIZ, DA SILVA EDER MARQUES, E SILVA GERALDO FELIPE FERREIRA, VALENTE GUILHERME TARGINO, BARRERA ROJAS CARLOS H: "Functional and evolutionary analyses of the miR156 and miR529 families in land plants", BMC PLANT BIOLOGY, BIOMED CENTRAL, LONDON, GB, vol. 16, no. 1, 1 December 2016 (2016-12-01), GB , pages 40, XP093149327, ISSN: 1471-2229, DOI: 10.1186/s12870-016-0716-5
ZIJU DAI, XINTAO WANG, QING YANG, FUCAI ZHANG, BAOQUAN LI: "Research Progresses of Differentiation and Development Genes of Tassel Branch Number of Maize(Zea mays L.)", JOURNAL OF AGRICULTURE, vol. 8, no. 10, 20 October 2018 (2018-10-20), pages 7 - 12, XP093149328
MOREA EDNA GICELA ORTIZ, DA SILVA EDER MARQUES, E SILVA GERALDO FELIPE FERREIRA, VALENTE GUILHERME TARGINO, BARRERA ROJAS CARLOS H: "Functional and evolutionary analyses of the miR156 and miR529 families in land plants", BMC PLANT BIOLOGY, BIOMED CENTRAL, LONDON, GB, vol. 16, no. 1, 1 December 2016 (2016-12-01), GB , pages 40, XP093149327, ISSN: 1471-2229, DOI: 10.1186/s12870-016-0716-5
ZIJU DAI, XINTAO WANG, QING YANG, FUCAI ZHANG, BAOQUAN LI: "Research Progresses of Differentiation and Development Genes of Tassel Branch Number of Maize(Zea mays L.)", JOURNAL OF AGRICULTURE, vol. 8, no. 10, 20 October 2018 (2018-10-20), pages 7 - 12, XP093149328
Attorney, Agent or Firm:
SHINY INTELLECTUAL PROPERTY FIRM (CN)
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