Title:
METHOD FOR REMOVING CARRYOVER CONTAMINATION IN NUCLEIC ACID AMPLIFICATION REACTIONS BY USING PHOTOBACTERIUM LEIOGNATHI-DERIVED URACIL-DNA GLYCOSYLASE
Document Type and Number:
WIPO Patent Application WO/2023/132543
Kind Code:
A1
Abstract:
The present invention relates to a uracil-DNA glycosylase (UDG) derived from a photobacterium leiognathi strain, and use thereof. A novel UDG of the present invention is an enzyme isolated from low-temperature bacteria photobacterium leiognathi, has an activity for decomposing a uracil base in a DNA substrate including uracil and is easily denatured at low temperature. Therefore, the UDG of the present invention allows carryover contamination occurring during nucleic acid amplification reactions to be removed, and thus can be effectively used for increasing accuracy (false positive elimination), purity and amplification efficiency of nucleic acid amplification reactions.
Inventors:
KWON SUK-TAE (KR)
NA HYEWON (KR)
CHO SUNG SUK (KR)
KWON CHANHO (KR)
NA HYEWON (KR)
CHO SUNG SUK (KR)
KWON CHANHO (KR)
Application Number:
PCT/KR2022/021237
Publication Date:
July 13, 2023
Filing Date:
December 23, 2022
Export Citation:
Assignee:
SEEGENE INC (KR)
International Classes:
C12Q1/6848; C07K1/16; C12N9/24; C12N15/52; C12N15/70
Foreign References:
| KR100787995B1 | 2007-12-24 | |||
| KR20080083428A | 2008-09-18 | |||
| KR20090039531A | 2009-04-22 |
Other References:
DATABASE Protein 18 January 2020 (2020-01-18), ANONYMOUS : "uracil-DNA glycosylase [Photobacterium leiognathi] ", XP093078037, retrieved from NCBI Database accession no. WP_023931008.1
ANUPAM SINGH; VAIBHAV UPADHYAY; ARUN KUMAR UPADHYAY; SURINDER MOHAN SINGH; AMULYA KUMAR PANDA: "Protein recovery from inclusion bodies of Escherichia coli using mild solubilization process", MICROBIAL CELL FACTORIES, SPRINGER, vol. 14, no. 1, 25 March 2015 (2015-03-25), pages 41, XP021220426, ISSN: 1475-2859, DOI: 10.1186/s12934-015-0222-8
VALLEJO LUIS FELIPE; RINAS URSULA: "Strategies for the recovery of active proteins through refolding of bacterial inclusion body proteins", MICROBIAL CELL FACTORIES, SPRINGER, vol. 3, no. 1, 2 September 2004 (2004-09-02), pages 11, XP021007184, ISSN: 1475-2859, DOI: 10.1186/1475-2859-3-11
LONGO M. C., BERNINGER M. S., HARTLEY J. L.: "USE OF URACIL DNA GLYCOSYLASE TO CONTROL CARRY-OVER CONTAMINATION IN POLYMERASE CHAIN REACTIONS.", GENE, ELSEVIER AMSTERDAM, NL, vol. 93., no. 01., 1 January 1990 (1990-01-01), NL , pages 125 - 128., XP000371626, ISSN: 0378-1119, DOI: 10.1016/0378-1119(90)90145-H
ANUPAM SINGH; VAIBHAV UPADHYAY; ARUN KUMAR UPADHYAY; SURINDER MOHAN SINGH; AMULYA KUMAR PANDA: "Protein recovery from inclusion bodies of Escherichia coli using mild solubilization process", MICROBIAL CELL FACTORIES, SPRINGER, vol. 14, no. 1, 25 March 2015 (2015-03-25), pages 41, XP021220426, ISSN: 1475-2859, DOI: 10.1186/s12934-015-0222-8
VALLEJO LUIS FELIPE; RINAS URSULA: "Strategies for the recovery of active proteins through refolding of bacterial inclusion body proteins", MICROBIAL CELL FACTORIES, SPRINGER, vol. 3, no. 1, 2 September 2004 (2004-09-02), pages 11, XP021007184, ISSN: 1475-2859, DOI: 10.1186/1475-2859-3-11
LONGO M. C., BERNINGER M. S., HARTLEY J. L.: "USE OF URACIL DNA GLYCOSYLASE TO CONTROL CARRY-OVER CONTAMINATION IN POLYMERASE CHAIN REACTIONS.", GENE, ELSEVIER AMSTERDAM, NL, vol. 93., no. 01., 1 January 1990 (1990-01-01), NL , pages 125 - 128., XP000371626, ISSN: 0378-1119, DOI: 10.1016/0378-1119(90)90145-H
Attorney, Agent or Firm:
KONG, Byung Wook (KR)
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