Title:
METHOD FOR REPLICATING OR AMPLIFYING CIRCULAR DNA
Document Type and Number:
WIPO Patent Application WO/2018/159669
Kind Code:
A1
Abstract:
Provided is a method that enables replication or amplification of circular DNA, particularly long-chain circular DNA, in a cell-free system. Specifically, provided is a method for inhibiting generation of a DNA multimer which is a by-product, during replication or amplification of circular DNA having a replication initiation sequence (origin of chromosome (oriC)) by using the following enzymes: (1) first enzymes which catalyze replication of the circular DNA; (2) second enzymes which catalyze an Okazaki fragment linking reaction, to synthesize two sister circular DNA molecules forming a catenane; and (3) third enzymes which catalyze a separation reaction of the two sister circular DNA molecules. Further provided is a method that comprises introduction of oriC into circular DNA by using a transposon.
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Inventors:
SU'ETSUGU MASAYUKI (JP)
NARA SEIA (JP)
NARA SEIA (JP)
Application Number:
PCT/JP2018/007485
Publication Date:
September 07, 2018
Filing Date:
February 28, 2018
Export Citation:
Assignee:
JAPAN SCIENCE & TECH AGENCY (JP)
International Classes:
C12N15/10; C12N15/11
Domestic Patent References:
| WO2016080424A1 | 2016-05-26 | |||
| WO2017199991A1 | 2017-11-23 |
Foreign References:
| JPH08510388A | 1996-11-05 | |||
| JP2003503029A | 2003-01-28 | |||
| JP2005229950A | 2005-09-02 | |||
| JP2008161182A | 2008-07-17 | |||
| JP2012501173A | 2012-01-19 |
Other References:
SU ' ETSUGU, M. ET AL.: "Exponential propagation of large circular DNA by reconstitution of a chromosome-replication cycle", NUCLEIC ACIDS RES., vol. 45, no. 20, September 2017 (2017-09-01), pages 11525 - 11534, XP055458551, ISSN: 0305-1048
HIASA, H. ET AL.: "Topoisomerase IV can support oriC DNA replication in vitro", J. BIOL. CHEM., vol. 269, no. 23, 1994, pages 16371 - 16375, XP055539849, ISSN: 0021-9258
FAKRUDDIN M ET AL., J PHARM BIOALLIED SCI., vol. 5, 2013, pages 245 - 252
PENG HMARIANS KJ, PNAS, vol. 90, 1993, pages 8571 - 8575
HIASA HMARIANS KJ., J BIOL CHEM., vol. 269, 1994, pages 26959 - 26968
FUNNELL B ET AL., J BIOL CHEM., vol. 261, 1986, pages 5616 - 5624
KAGUNI JMKORNBERG A, CELL, vol. 38, 1984, pages 183 - 90
HIASA, H.MARIANS, K. J., J. BIOL. CHEM., vol. 269, 1994, pages 26959 - 26968
NEYLON, C. ET AL., MICROBIOL. MOL. BIOL. REV., September 2005 (2005-09-01), pages 501 - 526
VIVIAN ET AL., J. MOL. BIOL., vol. 370, 2007, pages 481 - 491
IP, S. C. Y. ET AL., EMBO J., vol. 22, 2003, pages 6399 - 6407
COLLOMS ET AL., EMBO J., vol. 15, no. 5, 1996, pages 1172 - 1181
ARCISZEWSKA, L. K. ET AL., J. MOL. BIOL., vol. 299, 2000, pages 391 - 403
ALTSCHUL ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 10
SIEGEL, R. W. ET AL., FEBS LETT., vol. 499, no. 1-2, 2001, pages 147 - 153
ARAKI, K. ET AL., NUCLEIC ACIDS RES., vol. 25, no. 4, 1997, pages 868 - 872
GRINDLEY N. D. F. ET AL., CELL, vol. 30, no. 1, 1982, pages 227 - 234
ANASTASSIADIS, K. ET AL., DIS. MODEL. MECH., vol. 2, 2009, pages 508 - 515
ARAKI, H. ET AL., J. MOL. BIOL., vol. 182, no. 2, 1985, pages 191 - 203
SMITH, M. C. ET AL., MOL. MICROBIOL., vol. 44, 2002, pages 299
KAHMANN. R. ET AL., CELL, vol. 41, 1985, pages 771 - 780
GLASGOW. A. C. ET AL., J. BIOL. CHEM., vol. 264, 1989, pages 10072 - 10082
GORYSHIN, I. Y.REZNIKOFF, W. S., J. BIOL. CHEM., vol. 273, 1998, pages 7367 - 7374
See also references of EP 3591051A4
HIASA, H. ET AL.: "Topoisomerase IV can support oriC DNA replication in vitro", J. BIOL. CHEM., vol. 269, no. 23, 1994, pages 16371 - 16375, XP055539849, ISSN: 0021-9258
FAKRUDDIN M ET AL., J PHARM BIOALLIED SCI., vol. 5, 2013, pages 245 - 252
PENG HMARIANS KJ, PNAS, vol. 90, 1993, pages 8571 - 8575
HIASA HMARIANS KJ., J BIOL CHEM., vol. 269, 1994, pages 26959 - 26968
FUNNELL B ET AL., J BIOL CHEM., vol. 261, 1986, pages 5616 - 5624
KAGUNI JMKORNBERG A, CELL, vol. 38, 1984, pages 183 - 90
HIASA, H.MARIANS, K. J., J. BIOL. CHEM., vol. 269, 1994, pages 26959 - 26968
NEYLON, C. ET AL., MICROBIOL. MOL. BIOL. REV., September 2005 (2005-09-01), pages 501 - 526
VIVIAN ET AL., J. MOL. BIOL., vol. 370, 2007, pages 481 - 491
IP, S. C. Y. ET AL., EMBO J., vol. 22, 2003, pages 6399 - 6407
COLLOMS ET AL., EMBO J., vol. 15, no. 5, 1996, pages 1172 - 1181
ARCISZEWSKA, L. K. ET AL., J. MOL. BIOL., vol. 299, 2000, pages 391 - 403
ALTSCHUL ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 10
SIEGEL, R. W. ET AL., FEBS LETT., vol. 499, no. 1-2, 2001, pages 147 - 153
ARAKI, K. ET AL., NUCLEIC ACIDS RES., vol. 25, no. 4, 1997, pages 868 - 872
GRINDLEY N. D. F. ET AL., CELL, vol. 30, no. 1, 1982, pages 227 - 234
ANASTASSIADIS, K. ET AL., DIS. MODEL. MECH., vol. 2, 2009, pages 508 - 515
ARAKI, H. ET AL., J. MOL. BIOL., vol. 182, no. 2, 1985, pages 191 - 203
SMITH, M. C. ET AL., MOL. MICROBIOL., vol. 44, 2002, pages 299
KAHMANN. R. ET AL., CELL, vol. 41, 1985, pages 771 - 780
GLASGOW. A. C. ET AL., J. BIOL. CHEM., vol. 264, 1989, pages 10072 - 10082
GORYSHIN, I. Y.REZNIKOFF, W. S., J. BIOL. CHEM., vol. 273, 1998, pages 7367 - 7374
See also references of EP 3591051A4
Attorney, Agent or Firm:
ONO, Shinjiro et al. (JP)
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