Title:
METHOD FOR SAFELY PRODUCING BOTULINUM NEUROTOXIN
Document Type and Number:
WIPO Patent Application WO/2021/125866
Kind Code:
A1
Abstract:
The present invention relates to a method for producing botulinum toxin in various fragments to then be reassembled, for safely producing same. In the present invention, devised is a method in which: botulinum toxin is produced in fragments by cleaving light and heavy chains thereof into two or three pieces, respectively, and then combined as a full-length toxin, thereby allowing high complexity in production, due to toxicity, as well as low safety and economic feasibility, to be overcome; production of water-soluble botulinum toxin is enabled by using bacteria, thereby markedly shortening the production time as compared to existing production methods; and conjugation of the produced fragments with other proteins and nanoparticles is also enabled, thereby increasing the pharmaceutical extensibility of the toxin.
Inventors:
KWEON DAE-HYUK (KR)
PARK JOON-BUM (KR)
KIM YONG-JUN (KR)
PARK JOON-BUM (KR)
KIM YONG-JUN (KR)
Application Number:
PCT/KR2020/018623
Publication Date:
June 24, 2021
Filing Date:
December 18, 2020
Export Citation:
Assignee:
RESEARCH & BUSINESS FOUND SUNGKYUNKWAN UNIV (KR)
International Classes:
C12N15/70; A61K38/16; A61P25/00; C07K14/33
Domestic Patent References:
| WO2014110393A1 | 2014-07-17 |
Foreign References:
| US20060024794A1 | 2006-02-02 | |||
| US20130345398A1 | 2013-12-26 | |||
| KR20180130452A | 2018-12-07 | |||
| US20100196421A1 | 2010-08-05 |
Other References:
WEBER ET AL., CELL, vol. 92, 1998, pages 759 - 772
SINGH ET AL.: "Natural Toxins", vol. II, 1976, PLENUM PRESS, article "Critical Aspects of Bacterial Protein Toxins", pages: 63 - 84
ZHOU Y.SINGH B.R., PROTEIN EXPR. PURIF., vol. 34, 2004, pages 8 - 16
BAND P.A.BLAIS S.NEUBERT T.A.CARDOZO T.J.ICHTCHENKO K., PROTEIN EXPR. PURIF., vol. 71, 2010, pages 62 - 73
"Goodman and Gilman's The Pharmacological Basis of Therapeutics", 2001, PERGAMON PRESS
"Remington's Pharmaceutical Sciences", 1990, MACK PUBLISHING COMPANY
FERNANDEZ-SALAS E ET AL., PLOS ONE, vol. 7, no. 11, 2012, pages e49516
See also references of EP 4006158A4
SINGH ET AL.: "Natural Toxins", vol. II, 1976, PLENUM PRESS, article "Critical Aspects of Bacterial Protein Toxins", pages: 63 - 84
ZHOU Y.SINGH B.R., PROTEIN EXPR. PURIF., vol. 34, 2004, pages 8 - 16
BAND P.A.BLAIS S.NEUBERT T.A.CARDOZO T.J.ICHTCHENKO K., PROTEIN EXPR. PURIF., vol. 71, 2010, pages 62 - 73
"Goodman and Gilman's The Pharmacological Basis of Therapeutics", 2001, PERGAMON PRESS
"Remington's Pharmaceutical Sciences", 1990, MACK PUBLISHING COMPANY
FERNANDEZ-SALAS E ET AL., PLOS ONE, vol. 7, no. 11, 2012, pages e49516
See also references of EP 4006158A4
Attorney, Agent or Firm:
KONG, Byung Wook (KR)
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