Title:
METHOD FOR SCREENING CANCER THERAPEUTIC AGENT BY USING MODIFIED RARS PROTEIN
Document Type and Number:
WIPO Patent Application WO/2015/012559
Kind Code:
A1
Abstract:
The present invention relates to a method for screening a cancer therapeutic agent by using a modified RARS protein, and more specifically, to a method for screening a cancer therapeutic agent by using a modified RARS protein represented by SEQ ID NO: 4 in which 72 amino acids are removed from the N terminal of human-derived arginyl tRNA synthetase (RARS) protein, and the location of an amino acid moiety at which the protein binds to L-canavanine. The amino acid moiety of the modified RARS protein of the present invention which binds to canavanine is effective for screening a material, that is, a cancer therapeutic agent, capable of acting as a competitive inhibitor of arginine, such as canavanine.
More Like This:
Inventors:
HWANG KWANG YEON (KR)
KIM HYUN SOOK (KR)
KIM SUNGHOON (KR)
KIM HYUN SOOK (KR)
KIM SUNGHOON (KR)
Application Number:
PCT/KR2014/006627
Publication Date:
January 29, 2015
Filing Date:
July 22, 2014
Export Citation:
Assignee:
UNIV KOREA RES & BUS FOUND (KR)
MEDICINAL BIOCONVERGENCE RES CT (KR)
MEDICINAL BIOCONVERGENCE RES CT (KR)
International Classes:
C12N9/00; C07K16/40; C12N15/52; G01N33/15
Other References:
YANG, F. ET AL.: "Hemin Binds to Human Cytoplasmic Arginyl-tRNA Synthetase and Inhibits its Catalytic Activity.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 285, no. 50, 10 December 2010 (2010-12-10), pages 39437 - 39446
BENCE, A. K. ET AL.: "The Mechanism of L-Canavanine Cytotoxicity: Arginyl tRNA Synthetase as a Novel Target for Anticancer Drug Discovery.", JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, vol. 18, no. 5, October 2003 (2003-10-01), pages 383 - 394
ZHENG, Y. G. ET AL.: "Two Forms of Human Cytoplasmic Arginyl-tRNA Synthetase Produced from Two Translation Initiations by a Single mRNA.", BIOCHEMISTRY, vol. 45, no. 4, 31 January 2006 (2006-01-31), pages 1338 - 1344, XP055074355, DOI: doi:10.1021/bi051675n
LAZARD, M. ET AL.: "The tRNA-dependent Activation of Arginine by Arginyl-tRNA Synthetase Requires Inter-domain Communication.", JOURNAL OF MOLECULAR BIOLOGY, vol. 302, no. 4, 29 September 2000 (2000-09-29), pages 991 - 1004, XP004465939, DOI: doi:10.1006/jmbi.2000.4102
BENCE, A. K. ET AL.: "The Mechanism of L-Canavanine Cytotoxicity: Arginyl tRNA Synthetase as a Novel Target for Anticancer Drug Discovery.", JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, vol. 18, no. 5, October 2003 (2003-10-01), pages 383 - 394
ZHENG, Y. G. ET AL.: "Two Forms of Human Cytoplasmic Arginyl-tRNA Synthetase Produced from Two Translation Initiations by a Single mRNA.", BIOCHEMISTRY, vol. 45, no. 4, 31 January 2006 (2006-01-31), pages 1338 - 1344, XP055074355, DOI: doi:10.1021/bi051675n
LAZARD, M. ET AL.: "The tRNA-dependent Activation of Arginine by Arginyl-tRNA Synthetase Requires Inter-domain Communication.", JOURNAL OF MOLECULAR BIOLOGY, vol. 302, no. 4, 29 September 2000 (2000-09-29), pages 991 - 1004, XP004465939, DOI: doi:10.1006/jmbi.2000.4102
Attorney, Agent or Firm:
LEE, Hee Sook et al. (KR)
이희숙 (KR)
이희숙 (KR)
Download PDF: