Title:
METHOD FOR SCREENING KETOSIS-RESISTANT MOLECULAR MARKERS OF DAIRY COWS AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/248793
Kind Code:
A1
Abstract:
Provided are a method for screening ketosis-resistant molecular markers of dairy cows and an application thereof, which belong to the technical field of molecular genetic biology. Taking healthy and ketosis dairy cows as samples, chip sequencing, data filling, selection signal analysis, and whole-genome association analysis, and the like are performed to screen out a key gene APOA1 that affects the resistance of Chinese Holstein cattle to ketosis. The single nucleotide polymorphism site g.-572A>G is obtained by screening in the APOA1 gene promoter region. By means of association analysis, promoter activity analysis and other methods, it is obtained that the concentration of β-hydroxybutyric acid in the blood of GG genotype dairy cows is significantly lower than that of the AA genotype, and the promoter activity of GG genotype individuals is significantly higher than that of AA genotype individuals. By using the technical solution, genotyping the APOA1 gene promoter region site g.-572A>G may screen out dairy cow individuals that have strong ketosis resistance, thus achieving the purpose of saving on breeding costs.
Inventors:
HUANG JINMING (CN)
BAI JIACHEN (CN)
JU ZHIHUA (CN)
JIANG QIANG (CN)
WANG JINPENG (CN)
ZHANG YARAN (CN)
WANG XIUGE (CN)
WEI XIAOCHAO (CN)
GAO YAPING (CN)
YANG CHUNHONG (CN)
LIU WENHAO (CN)
LI RONGLING (CN)
GAO YUNDONG (CN)
BAI JIACHEN (CN)
JU ZHIHUA (CN)
JIANG QIANG (CN)
WANG JINPENG (CN)
ZHANG YARAN (CN)
WANG XIUGE (CN)
WEI XIAOCHAO (CN)
GAO YAPING (CN)
YANG CHUNHONG (CN)
LIU WENHAO (CN)
LI RONGLING (CN)
GAO YUNDONG (CN)
Application Number:
PCT/CN2020/126281
Publication Date:
December 16, 2021
Filing Date:
November 03, 2020
Export Citation:
Assignee:
DAIRY CATTLE RES CENTER SHANDONG ACADEMY OF AGRICULTURAL SCIENCE (CN)
SHANDONG OX LIVESTOCK BREEDING CO LTD (CN)
SHANDONG OX LIVESTOCK BREEDING CO LTD (CN)
International Classes:
C12Q1/6888
Domestic Patent References:
| WO2017058897A1 | 2017-04-06 |
Foreign References:
| CN111485027A | 2020-08-04 | |||
| CN110106250A | 2019-08-09 | |||
| CN109994153A | 2019-07-09 | |||
| CN109680078A | 2019-04-26 |
Other References:
BAI, JIACHEN: "Selection Signature Analysis and Gene Function Verification for Ketosis Resistance in Chinese Holstein Cattle", AGRICULTURE, CHINA MASTER’S THESES FULL-TEXT DATABASE, 15 August 2020 (2020-08-15), pages 1 - 84, XP009532755
YANG LING, JIACHEN BAI, ZHIHUA JU, QIANG JIANG, JINPENG WANG, YAPING GAO, YARAN ZHANG, XIAOCHAO WEI, JINMING HUANG: "Effect of functional single nucleotide polymorphism g.-572 A > G of apolipoprotein A1 gene on resistance to ketosis in Chinese Holstein cows", RESEARCH IN VETERINARY SCIENCE, vol. 135, 20 October 2020 (2020-10-20), pages 310 - 316, XP055878249, DOI: 10.1016/j.rvsc.2020.10.006
MAHMOUDI AHMAD, AMIR ZARGARAN, HAMID-REZA AMINI, ASSAD ASSADI, REZA VAJDI HOKMABAD, SHAHIN EGHBALSAIED : "A SNP in the 3′-untranslated region of AMPKγ1 may associate with serum ketone body and milk production of Holstein dairy cows", GENE, vol. 574, 27 July 2015 (2015-07-27), pages 48 - 52, XP055878251, DOI: 10.1016/j.gene.2015.07.077
HUANG H., J CAO, Q HANIF, Y WANG, Y YU, S ZHANG, Y ZHANG : "Genome-wide association study identifies energy metabolism genes for resistance to ketosis in Chinese Holstein cattle", INTERNATIONAL FOUNDATION FOR ANIMAL GENETICS, vol. 50, 31 December 2019 (2019-12-31), XP055878255, DOI: 10.1111/age.12802
YANG LING, JIACHEN BAI, ZHIHUA JU, QIANG JIANG, JINPENG WANG, YAPING GAO, YARAN ZHANG, XIAOCHAO WEI, JINMING HUANG: "Effect of functional single nucleotide polymorphism g.-572 A > G of apolipoprotein A1 gene on resistance to ketosis in Chinese Holstein cows", RESEARCH IN VETERINARY SCIENCE, vol. 135, 20 October 2020 (2020-10-20), pages 310 - 316, XP055878249, DOI: 10.1016/j.rvsc.2020.10.006
MAHMOUDI AHMAD, AMIR ZARGARAN, HAMID-REZA AMINI, ASSAD ASSADI, REZA VAJDI HOKMABAD, SHAHIN EGHBALSAIED : "A SNP in the 3′-untranslated region of AMPKγ1 may associate with serum ketone body and milk production of Holstein dairy cows", GENE, vol. 574, 27 July 2015 (2015-07-27), pages 48 - 52, XP055878251, DOI: 10.1016/j.gene.2015.07.077
HUANG H., J CAO, Q HANIF, Y WANG, Y YU, S ZHANG, Y ZHANG : "Genome-wide association study identifies energy metabolism genes for resistance to ketosis in Chinese Holstein cattle", INTERNATIONAL FOUNDATION FOR ANIMAL GENETICS, vol. 50, 31 December 2019 (2019-12-31), XP055878255, DOI: 10.1111/age.12802
Attorney, Agent or Firm:
JINAN SHENGDA INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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